Due to increasingly serious soil salinisation, exploring high‐quality closely related wild species is an effective means to solve food security problems. In this study, based on comprehensive metabolomics and transcriptomics analyses of the types, quantities, metabolic pathways and gene expression of small molecule metabolites in cotyledons and embryo axis/root, we report the strategies used by barren‐tolerant wild soybean (GS2) to resist salt stress during the post‐germination period. Our results showed that salt tolerance in GS2 cotyledons mainly involves the enhanced mobilisation of reserves, including lipid and sugar breakdown and utilisation, as well as protein breakdown and, in particular, the transport of amino acids to the embryo axis/root. Moreover, antioxidant capacity is enhanced through the promotion of ascorbic acid and naringin synthesis. We also found that under salt stress, the GS2 embryo axis/root accumulates proline by promoting the ornithine biosynthetic pathway, while stimulating glutathione metabolism to eliminate excess reactive oxygen species and restore oxidative balance. In addition, to establish and elongate the embryo axis/root, lignin synthesis is enhanced by the promotion of the shikimic acid pathway, which compensates for the decrease in cell wall support caused by salt stress. This study lays the foundation for developing and utilising high‐quality wild plant resources.

中文翻译：

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综合代谢组学和转录组学分析揭示了野生大豆（Glycine soja）在发芽后生长期间对盐胁迫的抵抗力


由于土壤盐碱化日益严重，探索优质近缘野生物种是解决粮食安全问题的有效手段。本研究基于对子叶和胚轴/根中小分子代谢物的类型、数量、代谢途径和基因表达的综合代谢组学和转录组学分析，报告了耐贫野生大豆（GS2）抵抗贫瘠的策略发芽后的盐胁迫。我们的结果表明，GS2子叶的耐盐性主要涉及储备动员的增强，包括脂质和糖的分解和利用，以及蛋白质分解，特别是氨基酸向胚轴/根的运输。此外，通过促进抗坏血酸和柚皮苷的合成来增强抗氧化能力。我们还发现，在盐胁迫下，GS2胚轴/根通过促进鸟氨酸生物合成途径积累脯氨酸，同时刺激谷胱甘肽代谢以消除过量的活性氧并恢复氧化平衡。此外，为了建立和延长胚轴/根，通过促进莽草酸途径增强木质素合成，这补偿了盐胁迫引起的细胞壁支持的减少。本研究为开发利用优质野生植物资源奠定了基础。