Aging is the process of gradual physio-biochemical deterioration. Although aging is inevitable, healthy aging is the key to individual and communal well-being. Therefore, it is essential to understand the regulation of aging. SIN-3/Sin-3 is a unique regulatory protein that regulates aging without DNA-binding activity. It functions by establishing multiple protein interactions. To understand the functional mechanism of this transcriptional regulator, the Caenorhabditis elegans protein interactome was assessed for SIN-3 interactions. DAF-16/FOXO emerged as one of the leading contenders for SIN-3-mediated regulation of aging. This study looks at the concerted role of SIN-3 and DAF-16 proteins in lifespan regulation. Phenotypic profiling for the mutants of these genes shows the functional accord between these 2 proteins with similar functions in stress response and vital biological processes. However, there were no significant physical interactions when checked for protein–protein interaction between SIN-3 and DAF-16 proteins. C. elegans genomics and transcriptomics data also indicated the possibilities of concerted gene regulation. This genetic regulation is more likely related to SIN-3 dominance on DAF-16 function. Overall, SIN-3 and DAF-16 proteins have strong functional interactions that ensure healthy aging. The influence of SIN-3 on DAF-16-mediated stress response is one of their convergence points in longevity regulation.

中文翻译：

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了解长寿：SIN-3 和 DAF-16 被揭示为寿命调节的独立参与者


衰老是逐渐生理生化恶化的过程。虽然老龄化是不可避免的，但健康老龄化是个人和社区福祉的关键。因此，了解衰老的调节至关重要。SIN-3/Sin-3 是一种独特的调节蛋白，可在没有 DNA 结合活性的情况下调节衰老。它通过建立多种蛋白质相互作用来发挥作用。为了了解这种转录调节因子的功能机制，评估了秀丽隐杆线虫蛋白相互作用组的 SIN-3 相互作用。DAF-16/FOXO 成为 SIN-3 介导的衰老调节的主要竞争者之一。本研究着眼于 SIN-3 和 DAF-16 蛋白在寿命调节中的协同作用。这些基因突变体的表型分析显示这 2 种蛋白质之间的功能一致性，在应激反应和重要生物过程中具有相似的功能。然而，当检查 SIN-3 和 DAF-16 蛋白之间的蛋白质-蛋白质相互作用时，没有明显的物理相互作用。秀丽隐杆线虫基因组学和转录组学数据也表明了协同基因调控的可能性。这种遗传调控更可能与 SIN-3 对 DAF-16 功能的优势有关。总体而言，SIN-3 和 DAF-16 蛋白具有很强的功能相互作用，可确保健康衰老。SIN-3 对 DAF-16 介导的应激反应的影响是它们在长寿调控中的收敛点之一。