OBJECTIVE To determine whether combining cross-linked (CL) collagen-integrated xenogeneic bone blocks stabilized with the fixation of resorbable collagen membranes (CM) can enhance guided bone regeneration (GBR) in the overaugmented calvarial defect model. MATERIALS AND METHODS Four circular defects with a diameter of 8 mm were prepared in the calvarium of 13 rabbits. Defects were randomly assigned to receive one of the following treatments: (i) non-cross-linked (NCL) porcine-derived collagen-embedded bone block covered by a CM without fixation (NCL + unfix group); (ii) NCL bone block covered by CM with fixation using bone-tack (NCL + fix group); (iii) cross-linked (CL) porcine-derived collagen-embedded bone block covered by CM without fixation (CL + unfix group); and (iv) CL bone block covered by CM with fixation using bone-tack fixation (CL + fix group). The efficacy of GBR was assessed through histological and molecular analyses after 2 and 8 weeks. RESULTS At 2 weeks, there were no significant differences in histologically measured areas of newly formed bone among the groups. At 8 weeks, however, the CL + fix group exhibited a larger area of new bone (5.08 ± 1.09 mm2, mean ± standard deviation) compared to the NCL + unfix (1.62 ± 0.42 mm2; p < .0083), NCL + fix (3.97 ± 1.39 mm2) and CL + unfix (2.55 ± 1.04 mm2) groups. Additionally, the expression levels of tumour necrosis factor-alpha, fibroblast growth factor-2, vascular endothelial growth factor, osteocalcin and calcitonin receptor were significantly higher in the CL + fix group compared to the other three groups (p < .0083). CONCLUSION Cross-linked bone blocks stabilized with collagen membrane fixation can significantly enhance GBR.

中文翻译：

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使用交联胶原蛋白结合的异种骨块和膜固定增强引导骨再生：一项临床前体内研究。


目的 确定结合交联 （CL） 胶原整合的异种骨块与可吸收胶原膜 （CM） 的固定是否可以增强过度增大颅骨缺损模型中的引导骨再生 （GBR）。材料与方法 在 13 只兔子的颅骨中制备了 4 个直径为 8 mm 的圆形缺陷。将缺损随机分配接受以下治疗之一：（i） 非交联 （NCL） 猪源性胶原包埋骨块被 CM 覆盖，无需固定（NCL + 未固定组）;（ii） CM 覆盖 NCL 骨块并使用骨粘性固定（NCL + 固定组）;（iii） 无固定的 CM 覆盖交联 （CL） 猪来源的胶原包埋骨块（CL + unfix 组）;（iv） CM 覆盖的 CL 骨块，使用骨粘性固定进行固定（CL + 固定组）。2 周和 8 周后通过组织学和分子分析评估 GBR 的疗效。结果 2 周时，各组新形成骨的组织学测量区域无显著差异。然而，在 8 周时，与 NCL + 未固定组 （1.62 ± 0.42 mm2;p < .0083）、NCL + 固定 （3.97 ± 1.39 mm2） 和 CL + 未固定 （2.55 ± 1.04 mm2） 组相比，CL + 固定组表现出更大的新骨面积 （5.08 ± 1.09 mm2，平均值±标准差）。此外，与其他三组相比，CL + 固定组肿瘤坏死因子-α、成纤维细胞生长因子-2、血管内皮生长因子、骨钙素和降钙素受体的表达水平显著升高 （p < .0083）。结论 胶原膜固定稳定的交联骨块可显著提高 GBR。