Tropomyosin was reported as an important allergen in Crassostrea angulata and designated as Cra a 1. The localization of the T cell epitopes and the reduction of the immunoreactivity of Cra a 1 are still lacking. In this study, four T cell epitopes were identified by using wild-type Cra a 1 (wtCra a 1)-immunized mouse splenocytes cultured with synthetic peptides. The immunoreactivity was maintained after chemical denaturation treatment, indicating that the linear epitope is an immunodominant epitope of wtCra a 1. Furthermore, the hypoallergenic derivative (mCra a 1) was developed by the deletion of linear B cell epitopes and retention of T cell epitopes. mCra a 1 could stimulate CD4⁺T cell proliferation and upregulate interleukin-10 secretion. Overall, basophil activation by mCra a 1 was low, but its ability to induce T cell proliferation was retained, suggesting that mCra a 1 may serve as a viable candidate for treating oyster allergy.

中文翻译：

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开发具有 B 细胞表位删除和 T 细胞表位保留的 Cra a 1 低变应原性衍生物


据报道，原肌球蛋白是Crassostrea angulata中的一种重要过敏原，并命名为 Cra a 1。T 细胞表位的定位和 Cra a 1 免疫反应性的降低仍然缺乏。在这项研究中，通过使用合成肽培养的野生型 Cra a 1 (wtCra a 1) 免疫小鼠脾细胞鉴定了四个 T 细胞表位。化学变性处理后仍保持免疫反应性，表明线性表位是wtCra a 1的免疫显性表位。此外，通过删除线性B细胞表位并保留T细胞表位开发了低变应原性衍生物（mCra a 1）。 mCra a 1 可以刺激 CD4 ⁺ T 细胞增殖并上调 IL-10 分泌。总体而言，mCra a 1 对嗜碱性粒细胞的激活较低，但其诱导 T 细胞增殖的能力得以保留，这表明 mCra a 1 可能作为治疗牡蛎过敏的可行候选药物。