This study investigated the uptake pathways, acropetal translocation, subcellular distribution, and biotransformation of OPEs by rice (Oryza sativa L.) after Cu exposure. The symplastic pathway was noted as the major pathway for the uptake of organophosphate triesters (tri-OPEs) and diesters (di-OPEs) by rice roots. Cu exposure enhanced the accumulation of tri-OPEs in rice roots, and such enhancement was positively correlated with Cu concentrations, attributing to the Cu-induced root damage. The hydrophilic Cl-OPEs in the cell-soluble fraction of rice tissues were enhanced after Cu exposure, while the subcellular distributions of alkyl- and aryl-OPEs were not affected by Cu exposure. Significantly higher biotransformation rates of tri-OPEs to di-OPEs occurred in leaves, followed by those in stems and roots. Our study reveals the mechanisms associated with the uptake, translocation, and biotransformation of various OPEs in rice after Cu exposure, which provides new insights regarding the phytoremediation of soils cocontaminated with heavy metal and OPEs.

中文翻译：

---

与铜共暴露会改变水稻 (Oryza sativa L.) 中有机磷酸三酯的吸收、积累、亚细胞分布和生物转化


本研究调查了水稻 ( Oryza sativa L.) 在铜暴露后对 OPE 的吸收途径、顶端易位、亚细胞分布和生物转化。共生途径被认为是水稻根部吸收有机磷酸三酯（tri-OPE）和二酯（di-OPE）的主要途径。 Cu暴露增强了水稻根部tri-OPE的积累，并且这种增强与Cu浓度呈正相关，归因于Cu引起的根部损伤。 Cu 暴露后，水稻组织细胞可溶部分中的亲水性 Cl-OPE 增强，而烷基和芳基-OPE 的亚细胞分布不受 Cu 暴露影响。 tri-OPEs 向 di-OPEs 的生物转化率在叶子中显着较高，其次是茎和根。我们的研究揭示了铜暴露后水稻中各种 OPE 的吸收、易位和生物转化的相关机制，这为重金属和 OPE 共同污染的土壤的植物修复提供了新的见解。