Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.

中文翻译：

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多光谱 3D 活体类器官成像平台，用于筛选用于荧光引导手术的探针。


实现肿瘤完全切除具有挑战性，可以通过使用分子靶向探针进行实时荧光引导手术来改善。然而，探针的临床前鉴定和验证是一个漫长的过程，传统上在动物模型中进行，并且进一步受到靶标表达的肿瘤间和肿瘤内异质性的阻碍。为了在患者规模筛选多个探针，我们开发了一个多光谱实时 3D 成像平台，该平台采用类器官技术来有效地模拟患者肿瘤异质性，重要的是，模拟健康的人体组织结合。