BACKGROUND Prolonged inflammation and oxidative stress can impede healing. To enhance healing efficiency, many solutions have been employed. This is an in vivo study comparing chlorhexidine (CHX) to a commercial antioxidant gel (AO). METHODS Envelope flaps were created in the lower incisor gingival region of 60 Sprague-Dawley rats, and acellular dermal matrix (ADM) was inserted. Animals were randomly assigned to postsurgical treatment application of AO gel or 0.12% CHX twice daily. A control group received no postsurgical treatment. Data collected (before surgery, 24 h, and 72 h) included surgical images, tissue samples, and weights. Blinded scorers assessed images using a wound healing scale. Real-time polymerase chain reaction (RT-PCR) was used for gene expression of tumor necrosis factor-alpha (TNFα), interleukin-1 (IL-1), myeloperoxidase (MPO), and superoxide dismutase (SOD). RESULTS The AO group scored higher than the CHX and control groups in clinical evaluation (p < 0.05). At 24 h, TNFα expression was upregulated in the AO group compared to CHX (p = 0.027) and controls (p = 0.018). The AO group had significantly higher expression of antioxidant enzyme (SOD) at 24 h compared to CHX (p = 0.021). All animals lost weight in the first 24 h. Animals treated with AO or CHX regained more weight at 72 h than control animals (p = 0.034 and 0.003, respectively). CONCLUSION Animals treated with AO healed faster. AO led to earlier upregulation of TNFα and antioxidant enzyme SOD. We hypothesized that AO promoted an earlier inflammatory process while counteracting oxidative stress by increasing antioxidant responses via SOD.

中文翻译：

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抗氧化凝胶与洗必泰在软组织愈合过程中的效果比较：一项动物研究。


背景技术长期的炎症和氧化应激会阻碍愈合。为了提高治疗效率，已经采用了许多解决方案。这是一项比较氯己定 (CHX) 和商业抗氧化凝胶 (AO) 的体内研究。方法 在 60 只 Sprague-Dawley 大鼠的下切牙牙龈区域制作包膜瓣，并插入脱细胞真皮基质 (ADM)。动物被随机分配接受 AO 凝胶或 0.12% CHX 的术后治疗，每天两次。对照组不接受术后治疗。收集的数据（手术前、24 小时和 72 小时）包括手术图像、组织样本和体重。盲法评分员使用伤口愈合量表评估图像。使用实时聚合酶链反应 (RT-PCR) 检测肿瘤坏死因子-α (TNFα)、白介素-1 (IL-1)、髓过氧化物酶 (MPO) 和超氧化物歧化酶 (SOD) 的基因表达。结果 AO 组在临床评价中得分高于 CHX 组和对照组（p < 0.05）。 24 小时时，与 CHX (p = 0.027) 和对照组 (p = 0.018) 相比，AO 组的 TNFα 表达上调。与 CHX 相比，AO 组在 24 小时时抗氧化酶 (SOD) 的表达显着较高 (p = 0.021)。所有动物在前 24 小时内体重减轻。用 AO 或 CHX 治疗的动物在 72 小时时比对照动物恢复了更多的体重（分别为 p = 0.034 和 0.003）。结论 使用 AO 治疗的动物愈合得更快。 AO 导致 TNFα 和抗氧化酶 SOD 的早期上调。我们假设 AO 促进早期炎症过程，同时通过 SOD 增加抗氧化反应来抵消氧化应激。