Propagation through cuttings is a well-established and effective technique for plant multiplication. This study explores the regeneration of poplar roots using spatial transcriptomics to map a detailed developmental trajectory. Mapping of the time-series transcriptome data revealed notable alterations in gene expression during root development, particularly in the activation of cytokinin-responsive genes. Our analysis identified six distinct clusters during the second and third stages, each corresponding to specific anatomical regions with unique gene expression profiles. Auxin response cis-elements (AuxREs) were prevalent in the promoters of these cytokinin-responsive genes, indicating a regulatory interplay between auxin and cytokinin. Pseudo-temporal trajectory analysis mapped the differentiation from cambium cells to root primordium cells, revealing a complex pattern of cell differentiation. SAC56 and LOS1 emerged as potential novel biomarkers for enhancing root regeneration, with distinct spatial expression patterns confirmed by in situ hybridization. This comprehensive spatial analysis enhances our understanding of the molecular interactions driving root regeneration and provides insights for improving plant propagation techniques.

中文翻译：

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空间转录组分析揭示杨树根的从头再生


通过插条繁殖是一种行之有效的植物繁殖技术。本研究利用空间转录组学探索杨树根的再生，绘制详细的发育轨迹。时间序列转录组数据的映射揭示了根发育过程中基因表达的显着变化，特别是在细胞分裂素响应基因的激活方面。我们的分析在第二和第三阶段确定了六个不同的簇，每个簇对应于具有独特基因表达谱的特定解剖区域。生长素反应顺式元件 (AuxRE) 在这些细胞分裂素反应基因的启动子中普遍存在，表明生长素和细胞分裂素之间存在调节相互作用。伪时间轨迹分析绘制了从形成层细胞到根原基细胞的分化，揭示了细胞分化的复杂模式。 SAC56 和 LOS1 成为增强根再生的潜在新型生物标志物，原位杂交证实其具有独特的空间表达模式。这种全面的空间分析增强了我们对驱动根再生的分子相互作用的理解，并为改进植物繁殖技术提供了见解。