Simultaneous two-color visualization of lipid droplets and lysosomes for cell homeostasis monitoring using a single fluorescent probe,Analyst

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Simultaneous two-color visualization of lipid droplets and lysosomes for cell homeostasis monitoring using a single fluorescent probe
Analyst ( IF 3.6 ) Pub Date : 2024-08-22 , DOI: 10.1039/d4an00912f
Mengxiao Liu ₁ , Yingcui Bu ₁ , Dongxiao Wang ₁ , Lihua Tang ₁ , Didi Hu ₁ , Longchun Li ₁ , Xiaoping Gan _{1,

2}

Affiliation

Lipid droplets (LDs) and lysosomes are vital organelles that play crucial roles in various physiological and pathological processes. However, simultaneous two-color visualization of these two organelles using a single probe for cell homeostasis monitoring remains a challenge due to the lack of rational design strategies. To address this issue, we have developed an aggregation-induced emission (AIE) fluorescent probe named TPE-NDI-Mor with an electron donor (D)–acceptor (A) structure, which can stain both LDs and lysosomes with high selectivity through green and red fluorescence imaging, respectively. A detailed mechanism study revealed that TPE-NDI-Mor, with a twisted intramolecular charge transfer (TICT) effect, shows a high affinity for a polar microenvironment. Additionally, the probe also demonstrates good stability, high anti-interference performance and a large Stokes shift, making it suitable for visualizing cell homeostasis and further disease diagnosis by tracking the dynamic changes of LDs and lysosomes.

中文翻译：

使用单个荧光探针同时对脂滴和溶酶体进行双色可视化，用于细胞稳态监测

脂滴（LD）和溶酶体是重要的细胞器，在各种生理和病理过程中发挥着至关重要的作用。然而，由于缺乏合理的设计策略，使用单个探针进行细胞稳态监测的同时双色可视化这两种细胞器仍然是一个挑战。为了解决这个问题，我们开发了一种名为TPE-NDI-Mor的聚集诱导发射（AIE）荧光探针，具有电子供体（D）-受体（A）结构，可以通过绿光对LD和溶酶体进行高选择性染色。和红色荧光成像，分别。详细的机制研究表明， TPE-NDI-Mor具有扭曲分子内电荷转移（TICT）效应，对极性微环境表现出高亲和力。此外，该探针还表现出良好的稳定性、高抗干扰性能和大的斯托克斯位移，使其适合通过跟踪LD和溶酶体的动态变化来可视化细胞稳态和进一步的疾病诊断。

更新日期：2024-08-22

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