Although protein subunit vaccines generally have acceptable safety profiles with precise antigenic content, limited immunogenicity can lead to unsatisfactory humoral and cellular immunity and the need for vaccine adjuvants and delivery system. Herein, we assess a vaccine adjuvant system comprising Quillaja Saponaria-21(QS-21) and cobalt porphyrin polymeric micelles that enabling the display of His-tagged antigen on its surface. The nanoscale micelles promote antigen uptake and dendritic cell activation to induce robust cytotoxic T lymphocyte response and germinal center formation. Using the recombinant protein antigens from influenza A and rabies virus, the micelle adjuvant system elicited robust antiviral responses and protected mice from lethal challenge. In addition, this system could be combined with other antigens to induce high titers of neutralizing antibodies in models of three highly pathogenic viral pathogens: Ebola virus, Marburg virus, and Nipah virus. Collectively, our results demonstrate this polymeric micelle adjuvant system can be used as a potent nanoplatform for developing antiviral vaccine countermeasures that promote humoral and cellular immunity.

中文翻译：

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一种广泛适用的抗病毒疫苗蛋白质聚合物佐剂系统。


尽管蛋白质亚单位疫苗通常具有可接受的安全性和精确的抗原含量，但有限的免疫原性可能导致体液和细胞免疫不令人满意，并且需要疫苗佐剂和递送系统。在此，我们评估了一种包含 Quillaja Saponaria-21（QS-21）和钴卟啉聚合物胶束的疫苗佐剂系统，该系统能够在其表面展示 His 标记的抗原。纳米级胶束促进抗原摄取和树突细胞活化，从而诱导强大的细胞毒性 T 淋巴细胞反应和生发中心形成。使用甲型流感病毒和狂犬病病毒的重组蛋白抗原，胶束佐剂系统引发了强大的抗病毒反应并保护小鼠免受致命攻击。此外，该系统可以与其他抗原结合，在三种高致病性病毒病原体模型中诱导高滴度的中和抗体：埃博拉病毒、马尔堡病毒和尼帕病毒。总的来说，我们的结果表明，这种聚合物胶束佐剂系统可以用作有效的纳米平台，用于开发促进体液和细胞免疫的抗病毒疫苗对策。