Cellular agriculture aims to meet the growing demand for animal products. However, current production technologies result in low yields, leading to economic projections that prohibit cultivated meat scalability. Here we use tangential flow filtration for continuous manufacturing of cultivated meat to produce biomass of up to 130 × 10⁶ cells per ml, corresponding to yields of 43% w/v and multiple harvests for over 20 days. Continuous manufacturing was carried out in an animal-component-free culture medium for US$0.63 l⁻¹ that supports the long-term, high density culture of chicken cells. Using this empirical data, we conducted a techno-economic analysis for a theoretical production facility of 50,000 l, showing that the cost of cultivated chicken can drop to within the range of organic chicken at US$6.2 lb⁻¹ by using perfusion technology. Whereas other variables would also affect actual market prices, continuous manufacturing can offer cost reductions for scaling up cultivated meat production.

细胞农业旨在满足对动物产品不断增长的需求。然而，当前的生产技术导致产量低，导致经济预测阻碍了养殖肉类的可扩展性。在这里，我们使用切向流过滤连续生产培养肉，产生每毫升高达 130 × 10 ^{6 个}细胞的生物量，相当于 43% w/v 的产量和 20 多天的多次收获。连续生产是在不含动物成分的培养基中进行的，价格为 0.63 l ^-1美元，支持鸡细胞的长期、高密度培养。利用这些经验数据，我们对50,000 l的理论生产设施进行了技术经济分析，结果表明，通过使用灌注技术，养殖鸡的成本可以降至6.2 lb ^-1美元的有机鸡的范围内。尽管其他变量也会影响实际市场价格，但连续生产可以降低养殖肉类生产规模的成本。