Radiation damage remains one of the major impediments to accurate structure solution in macromolecular crystallography. The artefacts of radiation damage can manifest as structural changes that result in incorrect biological interpretations being drawn from a model, they can reduce the resolution to which data can be collected and they can even prevent structure solution entirely. In this article, we discuss how to identify and mitigate against the effects of radiation damage at each stage in the macromolecular crystal structure-solution pipeline.

中文翻译：

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识别和避免大分子晶体学中的辐射损伤。


辐射损伤仍然是大分子晶体学中精确结构解析的主要障碍之一。辐射损伤的伪影可以表现为结构变化，导致从模型中得出不正确的生物学解释，它们可以降低数据收集的分辨率，甚至可以完全阻止结构求解。在本文中，我们讨论如何识别和减轻大分子晶体结构-解决方案流程中每个阶段的辐射损伤的影响。