Pyrocurzerenone is a natural compound found in Curcuma zedoaria and Chloranthus serratus. However, the anticancer effect of pyrocurzerenone in oral cancer remains unclear. Using the MTT assay, wound healing assay, transwell assay and western blot analysis, we investigated the impact of pyrocurzerenone on antimetastatic activity, as well as the critical signalling pathways that underlie the processes of oral cancer cell lines SCC‐9, SCC‐1 and SAS in this work. Our findings suggested that pyrocurzerenone inhibits cell migration and invasion ability in oral cancer cell lines. Furthermore, phosphorylation of ERK1/2 had significant inhibitory effects in SCC‐9 and SCC‐1 cell lines. Combining ERK1/2 inhibitors with pyrocurzerenone decreased the migration and invasion activity of SCC‐9 and SCC‐1 cell lines. We also found that the expressed level of cathepsin S decreased under pyrocurzerenone treatment. This study showed that pyrocurzerenone reduced ERK1/2 expression of the proteins and cathepsin S, suggesting that it could be a valuable treatment to inhibit human oral cancer cell metastasis.

中文翻译：

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Pyrocurzerenone 通过抑制 ERK1/2 和组织蛋白酶 S 蛋白的表达来抑制人口腔癌细胞转移


Pyrocurzerenone 是一种在姜黄和锯齿金粟中发现的天然化合物。然而，焦姜烯酮在口腔癌中的抗癌作用仍不清楚。使用 MTT 测定、伤口愈合测定、Transwell 测定和蛋白质印迹分析，我们研究了焦姜烯酮对抗转移活性的影响，以及口腔癌细胞系 SCC-9、SCC-1 和 SCC-1 过程中的关键信号通路。 SAS 在这项工作中。我们的研究结果表明，pyrocurzerenone 抑制口腔癌细胞系的细胞迁移和侵袭能力。此外，ERK1/2 的磷酸化对 SCC-9 和 SCC-1 细胞系具有显着的抑制作用。 ERK1/2 抑制剂与焦姜烯酮联合使用可降低 SCC-9 和 SCC-1 细胞系的迁移和侵袭活性。我们还发现在pyrocurzerenone处理下组织蛋白酶S的表达水平降低。这项研究表明，pyrocurzerenone 降低了 ERK1/2 蛋白和组织蛋白酶 S 的表达，表明它可能是抑制人类口腔癌细胞转移的有价值的治疗方法。