BackgroundThe field of drug development is witnessing a remarkable surge in the development of innovative strategies. There is a need to develop technological platforms capable of generating human data prior to progressing to clinical trials.MethodsHere we introduce a new flexible solution designed for the comprehensive monitoring of the natural human skin ecosystem's response to immunogenic drugs over time. Based on unique bioengineering to preserve surgical resections in a long survival state, it allows for the first time a comprehensive analysis of resident immune cells response at both organ and single‐cell levels.ResultsUpon injection of the mRNA‐1273 COVID‐19 vaccine, we characterized precise sequential molecular events triggered upon detection of the exogenous substance. The vaccine consistently targets DC/macrophages and mast cells, regardless of the administration route, while promoting specific cell–cell communications in surrounding immune cell subsets.ConclusionGiven its direct translational relevance, this approach provides a multiscale vision of genuine human tissue immunity that could pave the way toward the development of new vaccination and drug development strategies.

中文翻译：

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生物稳定人体皮肤模块的多模式分析揭示了对注射 mRNA-1273 COVID-19 疫苗的协调生态系统反应


背景药物开发领域正在见证创新战略开发的显着激增。需要开发能够在进入临床试验之前生成人类数据的技术平台。方法在这里，我们介绍了一种新的灵活解决方案，旨在全面监测自然人体皮肤生态系统对免疫原性药物的反应随时间的变化。基于独特的生物工程技术，可将手术切除保持长期生存状态，首次允许在器官和单细胞水平上对驻留免疫细胞反应进行全面分析。结果注射 mRNA-1273 COVID-19 疫苗后，我们表征了检测到外源性物质时触发的精确连续分子事件。无论给药途径如何，该疫苗始终以 DC/巨噬细胞和肥大细胞为靶点，同时促进周围免疫细胞亚群中的特定细胞间通讯。结论鉴于其直接的转化相关性，这种方法提供了真正的人体组织免疫的多尺度视野，这可能为开发新的疫苗接种和药物开发策略铺平道路。