Mitochondria play a key role in aging. Here, we measured integrated mitochondrial functions in experimentally evolved lines of the seed beetle Acanthoscelides obtectus that were selected for early (E) or late (L) reproduction for nearly 4 decades. The 2 lines have markedly different lifespans (8 days and 13 days in the E and L lines, respectively). The contribution of the NADH pathway to maximal flux was lower in the L compared to the E beetles at young stages, associated with increased control by complex I. In contrast, the contribution of the Succinate pathway was higher in the L than in the E line, whereas the Proline pathway showed no differences between the lines. Our data suggest that selection of age at reproduction leads to a modulation of complex I activity in mitochondria and that mitochondria are a functional link between evolutionary and mechanistic theories of aging.

中文翻译：

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选择晚期繁殖导致复合物 I 线粒体能力丧失，并导致种子甲虫的寿命延长


线粒体在衰老中起着关键作用。在这里，我们测量了种子甲虫 Acanthoscelides obtectus 的实验进化品系中的综合线粒体功能，这些品系被选择用于早期 （E） 或晚期 （L） 繁殖近 4 年。这 2 个系列的生命周期明显不同（E 系列和 L 系列分别为 8 天和 13 天）。与年轻阶段的 E 甲虫相比，L 的 NADH 通路对最大通量的贡献较低，这与复合物 I 的控制增加有关。相比之下，琥珀酸通路在 L 系中的贡献高于 E 系，而 Proline 通路在各系之间没有差异。我们的数据表明，生殖年龄的选择导致线粒体中复合物 I 活性的调节，并且线粒体是衰老的进化论和机械论之间的功能联系。