The beneficial effects of xylo-oligosaccharides (XOS) on the intestine have been widely reported, including anti-inflammation, antioxidant, maintenance of intestinal epithelial barrier, and treatment of intestinal injury. However, the specific mechanism of XOS in mitigating intestinal injury in weaned piglets remains unclear. Therefore, this study aimed to explore the specific mechanism of XOS in mitigating intestinal injury. The study is a complete randomized design with 24 weaned piglets in a 2 × 2 factorial arrangement that includes diet treatments (basal diet vs. 0.02% XOS) and immunological challenge [saline vs. lipopolysaccharide (LPS)]. All piglets were fed a basal diet or a XOS diet for 21 d. On day 22, all piglets received an injection of LPS or saline. In this study, dietary XOS increased jejunal villus height, reduced crypt depth and oxidative stress, and enhanced the gene and protein expression of Claudin-1, Occludin, and zonula occludens 1 (P < 0.05). The piglets fed the XOS diet had lower serum Diamine oxidase activity and d-lactic acid content (P < 0.05). In addition, dietary XOS regulates endoplasmic reticulum (ER)–mitochondria system function and the expression of key molecules, including mitochondrial dynamics dysfunction [mitofusin (Mfn)-1, optic atrophy 1, fission 1, and dynamin-related protein 1], ER stress [activating transcription factor 4 (ATF4), ATF6, C/EBP-homologous protein, eukaryotic initiation factor 2α, glucose-regulated protein (GRP) 78, GRP94, and protein kinase R-like ER kinase] and the mitochondria-associated ER membranes (MAM) disorders (Mfn2, GRP75, and voltage-dependent anion channel 1) (P < 0.05). Therefore, the findings to indicate that dietary XOS is effective against LPS-induced jejunal injury may be attributed to its ability to alleviate mitochondrial dynamics dysfunction, ER stress, and MAM disorders.

中文翻译：

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日粮低聚木糖通过仔猪内质网-线粒体系统途径减轻 LPS 诱导的肠道损伤


木质低聚糖 （XOS） 对肠道的有益作用已被广泛报道，包括抗炎、抗氧化、维持肠上皮屏障和治疗肠道损伤。然而，XOS 减轻断奶仔猪肠道损伤的具体机制仍不清楚。因此，本研究旨在探讨 XOS 减轻肠道损伤的具体机制。该研究是一项完整的随机设计，以 2 × 2 因子排列 24 头断奶仔猪，包括饮食处理（基础饮食与 0.02% XOS）和免疫激发 [盐水与脂多糖 （LPS）]。所有仔猪饲喂基础日粮或 XOS 日粮 21 d。第 22 天，所有仔猪都接受了 LPS 或生理盐水注射。在本研究中，饮食 XOS 增加了空肠绒毛高度，降低了隐窝深度和氧化应激，并增强了 Claudin-1、Occludin 和 zonula occludens 1 的基因和蛋白表达 （P < 0.05）。饲喂 XOS 日粮的仔猪血清二胺氧化酶活性和 d-乳酸含量较低 （P < 0.05）。此外，饮食 XOS 调节内质网 （ER）-线粒体系统功能和关键分子的表达，包括线粒体动力学功能障碍 [线粒体融合蛋白 （Mfn）-1、视神经萎缩 1、裂变 1 和动力蛋白相关蛋白 1]、ER 应激 [激活转录因子 4 （ATF4）、ATF6、C/EBP 同源蛋白、真核起始因子 2α、葡萄糖调节蛋白 （GRP） 78、GRP94 和蛋白激酶 R 样 ER 激酶] 和线粒体相关 ER 膜（MAM） 疾病 （Mfn2、GRP75 和电压依赖性阴离子通道 1） （P < 0.05）。 因此，表明膳食 XOS 对 LPS 诱导的空肠损伤有效的研究结果表明，其可能归因于其缓解线粒体动力学功能障碍、ER 应激和 MAM 疾病的能力。