The solid culture method for measuring the efficiency of ultraviolet (UV) disinfection of airborne bacteria is time-consuming, typically taking 12–48 h. To expedite such experiments, this study proposed a liquid culture method assisted by adenosine triphosphate (ATP) analysis, as a liquid culture is faster than a solid culture, and measurement of ATP does not require waiting for visible colonies to form. () was used as the experimental bacterium. This study first compared the log reduction of bacteria in liquid as measured by the proposed method and by the traditional solid culture method. The minimum liquid culture time was determined for different bacterial concentration ranges. Finally, the feasibility of the proposed method was validated by UV disinfection experiments on airborne bacteria. The results indicated that the proposed method measured a similar log reduction to that of the solid culture method in liquid experiments. The minimum liquid culture time for in 10-10 colony forming units (CFU)/mL was 2 h. The validation experiments demonstrated that the proposed method is capable of measuring the UV disinfection efficiency of airborne bacteria. The proposed method can accelerate laboratory experiments on UV disinfection of airborne bacteria, which in turn can support the effective design and utilization of UV disinfection in real life.

中文翻译：

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ATP分析辅助液体培养方法加速空气细菌紫外线消毒实验室实验


用于测量空气细菌紫外线 (UV) 消毒效率的固体培养方法非常耗时，通常需要 12-48 小时。为了加速此类实验，本研究提出了一种辅以三磷酸腺苷 (ATP) 分析的液体培养方法，因为液体培养比固体培养更快，并且 ATP 的测量不需要等待可见菌落形成。 ()被用作实验细菌。本研究首先比较了采用该方法和传统固体培养方法测量的液体中细菌的对数减少量。确定不同细菌浓度范围的最短液体培养时间。最后，通过紫外线对空气细菌的消毒实验验证了该方法的可行性。结果表明，所提出的方法在液体实验中测得与固体培养方法相似的对数减少。 10-10 菌落形成单位 (CFU)/mL 的最短液体培养时间为 2 小时。验证实验表明，该方法能够测量空气中细菌的紫外线消毒效率。该方法可以加速空气细菌紫外线消毒的实验室实验，从而支持现实生活中紫外线消毒的有效设计和使用。