Green synthesized iron/manganese nanoparticles (Fe/Mn NPs), acted as an exogenous promoter to enhance the lignin-degrading bacteria FJ17 resulting in more efficient removal of bisphenol A (BPA). Batch experiments demonstrated that removal efficiency of BPA via cells at a BPA concentration of 10 mg·L increased by 20.9 % when exposed to 100 mg·L Fe/Mn NPs after 48 h (93.63 %) relative to an unexposed control group (72.70 %). TEM and 3D-EEM analysis confirmed that the cell membrane thickness increased from 47 to 80 nm under Fe/Mn NPs exposure, and the TB-EPS secretion was promoted. Meanwhile, Fe/Mn NPs facilitated greater electron transfer capacity of -cytochrome (0.55 V reduction peak) and an unknown cytochrome substance (0.7 V oxidation peak) on the surface of cells. Studies of the effect of Fe/Mn NPs on both the growth and activity of laccase cells showed that both biomass and laccase secretion increased significantly during the logarithmic growth period (6–36 h). LC-MS analysis and toxicity assessment indicated that Fe/Mn NPs decreased the degradation time of BPA and efficiently reduced the toxicity of its by-products. Transcriptomic analysis revealed 315 up-regulation of the key genes associated with energy supply, membrane translocation, and metabolic pathways upon exposure to Fe/Mn NPs. Such as MFS transporter (2.27-fold), diguanylate cyclase (1.76-fold) and protocatechuate-3,4-dioxygenase (1.62-fold). Overall, Fe/Mn NPs accelerated proliferation by enhancing metabolic capacity and nutrient transport processes, which serves to improve the efficiency of BPA removal.

中文翻译：

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对 Fe/Mn 纳米粒子利用睾丸酮丛毛单胞菌促进双酚 A 去除的调节机制有了新的认识


Green 合成的铁/锰纳米粒子 (Fe/Mn NPs) 作为外源促进剂增强木质素降解细菌 FJ17，从而更有效地去除双酚 A (BPA)。批量实验表明，当BPA浓度为10 mg·L时，100 mg·L Fe/Mn NPs暴露48 h后，细胞对BPA的去除率提高了20.9 %（93.63 %），相对于未暴露的对照组（72.70 %） ）。 TEM和3D-EEM分析证实，Fe/Mn NPs暴露下细胞膜厚度从47 nm增加到80 nm，并且促进了TB-EPS的分泌。同时，Fe/Mn NPs促进了细胞表面的β细胞色素（0.55 V还原峰）和未知细胞色素物质（0.7 V氧化峰）更大的电子传递能力。 Fe/Mn NPs 对漆酶细胞生长和活性影响的研究表明，在对数生长期（6-36 h），生物量和漆酶分泌量均显着增加。 LC-MS分析和毒性评估表明，Fe/Mn NPs缩短了BPA的降解时间，并有效降低了其副产物的毒性。转录组分析显示，接触 Fe/Mn NP 后，与能量供应、膜易位和代谢途径相关的关键基因有 315 上调。如MFS转运蛋白（2.27倍）、二鸟苷酸环化酶（1.76倍）和原儿茶酸3,4-双加氧酶（1.62倍）。总体而言，Fe/Mn NPs 通过增强代谢能力和营养物质运输过程来加速增殖，从而提高 BPA 的去除效率。