Novel microbial strains capable of efficient degradation of TNT and typical intermediates (2-ADNT and 4-ADNT) in aerobic/anaerobic environment were screened and isolated from ammunition-contaminated sites. The key genomes, transcriptomes, proteins, and metabolic factors for microbial detoxification/tolerance to pollutants in anaerobic and aerobic environments were analyzed for the first time. The bacterial genome, which is rich in metabolism and environmental information-processing functional genes, provides transcriptional and translational-related proteins for detoxifying/tolerating pollutants. At the transcriptional level, bacteria significantly expressed genes related to inositol phosphate metabolism for regulating membrane transport, maintaining the cytoskeleton, and signal transduction. At the protein level, genes involved in antioxidation, fat metabolism, sugar synthesis/degradation, and pyruvate metabolism were significantly expressed. At the metabolic level, riboflavin metabolism, which regulates membrane integrity, protects against oxidative stress, and maintains the sugar–protein–fat balance, showed significant responses. Bacteria simultaneously regulate amino acid metabolism, carbohydrate metabolism, and N/P/S cycles to maintain homeostatic cellular energy supplies. The key pathway for pollutant degradation in bacteria is nitrotoluene degradation. The molecular mechanism of bacterial tolerance to pollutants involves the regulation of oxidative phosphorylation and basic cycle pathways to maintain gene transcription, protein translation, and metabolic cycles.

中文翻译：

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厌氧/需氧环境中细菌对 TNT 及其中间体的解毒/耐受所涉及的关键基因组、转录组、蛋白质和代谢因子


从弹药污染场地筛选分离出能够在好氧/厌氧环境下高效降解TNT和典型中间体（2-ADNT和4-ADNT）的新型微生物菌株。首次分析了厌氧和好氧环境下微生物解毒/耐受污染物的关键基因组、转录组、蛋白质和代谢因子。细菌基因组富含代谢和环境信息处理功能基因，为解毒/耐受污染物提供转录和翻译相关蛋白。在转录水平上，细菌显着表达与磷酸肌醇代谢相关的基因，以调节膜运输、维持细胞骨架和信号转导。在蛋白质水平上，参与抗氧化、脂肪代谢、糖合成/降解、丙酮酸代谢的基因显着表达。在代谢水平上，调节膜完整性、防止氧化应激并维持糖-蛋白质-脂肪平衡的核黄素代谢表现出显着的反应。细菌同时调节氨基酸代谢、碳水化合物代谢和 N/P/S 循环，以维持细胞能量供应的稳态。细菌降解污染物的关键途径是硝基甲苯降解。细菌对污染物耐受的分子机制涉及氧化磷酸化和基本循环途径的调节，以维持基因转录、蛋白质翻译和代谢循环。