To identify microbial resources for dehalogenation, develop effective remediation strategies, and reveal their significance in “One Health”, it is crucial to understand the occurrence, distribution, and drivers of soil dehalogenation functional traits and taxonomy groups at a broad scale, which is currently not well understood. To address the gaps, we characterized the biogeography of both dehalogenation traits and taxa assigned to six dehalogenation pathways, by metagenomic profiling global 4821 soils from eight habitats, based on a manually curated dehalogenase database (DhgaseDB). We found dehalogenation genes and microbes assigned to different pathways are everywhere, but varied consistently across habitats. The similarity of dehalogention traits and taxa composition declines with geographic distance, and that patterns are strongly correlated with geo-environmental factors. We identified anthropogenic organohalide pesticide inputs as the most influential factor on dehalogenation gene abundance, while soil properties, particularly pH, exert a larger impact on dehalogenation taxa diversity. Ultimately, we generated predictive maps of soil dehalogenation gene abundance and taxa diversity for the first time, highlighting the microbial dehalogenation hotpots in East Asia, Australia, Southern Africa, and coastal regions. Collectively, our study highlights the significant role of various microbial dehalogenation processes in organohalide biotransformation and environmental microecology, providing the necessary methodological basis for a deeper comprehension of the underlying mechanisms, thereby contributing to the advancement of tailored strategies for organohalide remediation.

中文翻译：

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解决全球生物地理学和土壤微生物脱卤性状和类群的驱动因素：基于精心策划的脱卤酶数据库的宏基因组分析的见解


为了识别脱卤微生物资源、制定有效的修复策略并揭示其在“同一个健康”中的意义，至关重要的是在大范围内了解土壤脱卤功能性状和分类群的发生、分布和驱动因素。不太理解。为了弥补这一差距，我们基于手动管理的脱卤酶数据库 (DhgaseDB)，通过对来自 8 个栖息地的全球 4821 个土壤进行宏基因组分析，描述了分配给 6 个脱卤途径的脱卤性状和类群的生物地理学特征。我们发现分配给不同途径的脱卤基因和微生物无处不在，但在不同的栖息地之间却存在差异。脱卤性状和类群组成的相似性随着地理距离的增加而下降，并且这种模式与地理环境因素密切相关。我们确定人为有机卤化物农药输入是对脱卤基因丰度影响最大的因素，而土壤特性，特别是pH值，对脱卤类群多样性影响更大。最终，我们首次生成了土壤脱卤基因丰度和类群多样性的预测图，突出显示了东亚、澳大利亚、南部非洲和沿海地区的微生物脱卤热点。总的来说，我们的研究强调了各种微生物脱卤过程在有机卤化物生物转化和环境微生态学中的重要作用，为更深入地理解潜在机制提供了必要的方法学基础，从而有助于推进有机卤化物修复的定制策略。