Endocrine-disrupting chemicals (EDCs) exhibited the detriment in female reproductive health. Our objective was to investigate the individual and mixture effects of EDCs present in follicular fluid, the environment in which oocytes grow and develop, on early reproductive outcomes. We recruited 188 women seeking reproduction examination from the Study of Exposure and Reproductive Health (SEARCH) cohort between December 2020 and November 2021. We assessed the concentrations of 7 categories of 64 EDCs in follicular fluid, and measured early reproductive outcomes, including retrieved oocytes, mature oocytes, normal fertilized oocytes, and high-quality embryos. In this study Monomethyl phthalate (MMP) (2.17 ng/ml) were the compounds found in the highest median concentrations in follicular fluid. After adjusting for multiple testing, multivariate regression showed that multiple EDCs were significantly negatively associated with early assisted reproduction outcomes. For example, MMP showed a significant negative correlation with the number of high quality embryos (β: −0.1, 95 % CI: −0.15, −0.04). Specifically, eight types of EDCs were significantly negatively associated with four early assisted reproductive outcomes (β range: −0.2 ∼ -0.03). In the mixed exposure model, we found that mixtures of EDC were significantly negatively correlated with all four outcomes. In the quantile g-computation (QGCOMP) model, for each interquartile range increase in the concentration of EDC mixtures, the number of oocytes retrieved, mature oocytes, normally fertilized oocytes, and high-quality embryos decreased by 0.46, 0.52, 0.77, and 1.2, respectively. Moreover, we identified that phthalates (PAEs) predominantly contributed to the negative effects. Future research should validate our findings.

中文翻译：

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卵泡液中内分泌干扰化学物质的影响：从卵母细胞到受精的见解


内分泌干​​扰化学物质（EDC）对女性生殖健康有害。我们的目标是研究卵泡液（卵母细胞生长和发育的环境）中存在的 EDC 对早期生殖结果的单独和混合影响。我们在 2020 年 12 月至 2021 年 11 月期间从暴露与生殖健康研究 (SEARCH) 队列中招募了 188 名寻求生殖检查的女性。我们评估了卵泡液中 7 类 64 种 EDC 的浓度，并测量了早期生殖结果，包括回收的卵母细胞、成熟卵母细胞、正常受精卵母细胞和高质量胚胎。在这项研究中，邻苯二甲酸单甲酯 (MMP) (2.17 ng/ml) 是卵泡液中中位浓度最高的化合物。调整多重测试后，多元回归显示多种 EDC 与早期辅助生殖结果显着负相关。例如，MMP与高质量胚胎的数量呈显着负相关（β：-0.1，95％CI：-0.15，-0.04）。具体而言，八种类型的 EDC 与四种早期辅助生殖结果显着负相关（β 范围：-0.2 ∼ -0.03）。在混合暴露模型中，我们发现 EDC 混合物与所有四种结果均显着负相关。在分位数 g 计算 (QGCOMP) 模型中，EDC 混合物浓度每增加一个四分位数，取出的卵母细胞、成熟卵母细胞、正常受精卵母细胞和高质量胚胎的数量分别减少 0.46、0.52、0.77 和分别为1.2。此外，我们发现邻苯二甲酸盐 (PAE) 是造成负面影响的主要原因。未来的研究应该验证我们的发现。