ObjectiveTo investigate the association between interleukin‐8 (IL‐8) levels in gingival crevicular fluid (GCF) and total oral fluid (TOF) and the responsiveness to steps 1 and 2 of periodontal therapy.Materials and MethodsOne‐hundred and fifty‐nine patients affected by periodontitis received steps 1 and 2 of periodontal therapy. At baseline, TOF and GCF samples were collected and analysed for IL‐8 (Il‐8TOF/IL‐8GCF) using flow cytometry. Therapy outcomes were relative proportions of residual periodontal pockets (PPD%), pocket closure (PC) rates and pocket probing depth (PPD) reductions; these were associated with IL‐8TOF/IL‐8GCF.ResultsHigh IL‐8TOF was significantly associated with higher residual PPD% (p = 0.044) and lower PPD reduction compared to low IL‐8TOF (high 0.79 ± 1.20 mm vs. low 1.20 ± 1.20 mm, p < 0.001) in non‐smokers, while in smokers high IL‐8GCF was related to lower PPD reduction (high 0.62 ± 1.22 mm vs. low 0.84 ± 1.12 mm, p = 0.009). Furthermore, high baseline IL‐8TOF was significantly associated with poorer PC rates compared to medium and low concentrations in both non‐smokers (high 41% vs. medium 55% vs. low 58%, p < 0.001) and smokers (high 34% vs. medium 44% vs. low 46%, p < 0.001).ConclusionHigh IL‐8 concentrations at baseline had a significant impact on residual PPD%, PC rates and PPD reduction. The findings suggest that, especially in non‐smokers, baseline IL‐8 levels collected from the TOF could serve as a component in the estimation of responsiveness to steps 1 and 2 of periodontal therapy.

中文翻译：

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白细胞介素 8 在估计对牙周治疗第 1 步和第 2 步的反应性中的作用


目的探讨牙龈沟液 （GCF） 和总口腔液 （TOF） 中白细胞介素-8 （IL-8） 水平与牙周治疗第 1 步和第 2 步反应性之间的相关性。材料和方法159 例受牙周炎影响的患者接受了第 1 步和第 2 步牙周治疗。基线时，收集 TOF 和 GCF 样品并使用流式细胞术分析 IL-8 （Il-8TOF/IL-8GCF）。治疗结果是残留牙周袋 （PPD%） 、牙周袋闭合 （PC） 率和牙周袋探诊深度 （PPD） 减少的相对比例;这些与 IL-8TOF/IL-8GCF 相关。结果与低 IL-8TOF 相比，高 IL-8TOF 与较高的残余 PPD 百分比 （p = 0.044） 和较低的 PPD 降低显著相关 PPD（高 0.79 ± 1.20 毫米与低 1.20 ± 1.20 毫米，p < 0.001）相比，在非吸烟者中，高 IL-8GCF 与较低的 PPD 降低（高 0.62 ± 1.22 毫米与低 0.84 ± 1.12 毫米）相关， p = 0.009）。此外，与中浓度和低浓度相比，非吸烟者（高 41% vs. 中 55% vs. 低 58%，p < 0.001）和吸烟者（高 34% vs. 中 44% vs. 低 46%，p < 0.001）与较差的 PC 率显著相关。结论基线时高 IL-8 浓度对残余 PPD、PC 率和 PPD 降低有显著影响。研究结果表明，特别是在非吸烟者中，从 TOF 收集的基线 IL-8 水平可以作为估计对牙周治疗第 1 步和第 2 步反应性的一个组成部分。