Mouse kidney transplantation provides a powerful preclinical model for the study of kidney transplant alloimmunity. However, accurate measurement of graft function is difficult because of the inaccuracy of traditional surrogate markers serum creatinine and urea. We report the use of transdermal glomerular filtration rate measurement under the experimental conditions of unilateral nephrectomy and allogeneic kidney transplantation. Our findings demonstrate that transdermal glomerular filtration rate measurement is easy to perform, reproducible, and has more interexperimental consistency than serum creatinine or urea measurements. Most importantly, it significantly reduces the numbers of experimental animals required to detect subtle and yet clinically relevant differences in kidney function as often is the case in experimental murine kidney transplantation models.

中文翻译：

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小鼠肾移植结局最好通过经皮肾小球滤过率来衡量


小鼠肾移植为肾移植同种免疫研究提供了强大的临床前模型。然而，由于传统替代标志物血清肌酐和尿素的不准确，移植物功能的准确测量很困难。我们报道了在单侧肾切除术和同种异体肾移植实验条件下经皮肾小球滤过率测量的使用。我们的研究结果表明，经皮肾小球滤过率测量易于执行、可重复，并且比血清肌酐或尿素测量具有更多的实验间一致性。最重要的是，它显着减少了检测肾功能细微但临床相关差异所需的实验动物数量，这在实验性小鼠肾移植模型中经常出现。