The primary objective of this study was to assess the feasibility of utilizing urine-prepared NaOH solution (NUr) as an innovative pretreatment approach for enhancing BHP in rice straw through anaerobic digestion (AD) process. The findings suggest that pretreatment with NUr could substantially enhance hydrolysis rates and H production. The maximum biohydrogen production (BHP) yield reached 184.46 mL/g substrate with 86.98 % holocellulose retention yield and 59.84 % removal of lignin when RS was pretreated by NUr at a concentration of 3 % w/v, 20 % solid loading, −12 °C, and 3 months. Macro and microstructure analysis confirmed more cellulose and hemicellulose were exposed due to the lignin removed, and crystallinity decreased after NUr pretreatment. The energy conversion efficiency (ECE) attained via NUr pretreated RS was 10.38 %, exceeded that of raw RS by 62.19 %. These findings offer a promising and efficient pretreatment technology for enhancing the recovery of H from lignocellulosic biomass, yielding both environmental and economic advantages.

中文翻译：

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NaOH-尿液预处理稻草提高生物氢产量：全纤维素保留率提升


本研究的主要目的是评估利用尿液制备的 NaOH 溶液 (NUr) 作为创新预处理方法通过厌氧消化 (AD) 过程提高稻草中 BHP 的可行性。研究结果表明，用 NUR 预处理可以显着提高水解速率和 H 产量。当RS经浓度为3 % w/v、20 %固体负载、-12 °的NUr预处理时，最大生物氢产量（BHP）达到184.46 mL/g底物，全纤维素保留率86.98 %，木质素去除率59.84 % C、3个月。宏观和微观结构分析证实，由于木质素被去除，更多的纤维素和半纤维素被暴露，并且NUr预处理后结晶度降低。经过NUr预处理的RS获得的能量转换效率（ECE）为10.38%，比原始RS高出62.19%。这些发现提供了一种有前途且高效的预处理技术，可提高木质纤维素生物质中氢气的回收率，从而产生环境和经济优势。