The conflict between macroporous structure and high specific surface area strongly limited the dynamic adsorption performance of chromatographic media. Here, macroporous and high-specific-surface-area cellulose microspheres (PCMs) are prepared by using semi-dissolved chitosan as a dual-functional template and subsequently functionalized with diethylaminoethyl (DEAE) as anion exchangers for protein separation. The dual-functional template strategy leverages dissolved chitosan nanofibers as meso/micropore templates and the undissolved chitosan particles as macropore templates. The macropores of DEAE-PCMs (average size: 0.4–1.2 μm) efficiently decrease mass transfer resistance for fast uptake kinetics. The high specific surface area (272.34 m/g) provides sufficient ligand sites for ensuring exceptional adsorption capacities. Therefore, the chitosan bifunctional templated PCMs, possessing a macro/meso/microporous structure, are promising candidates for high-performance chromatographic media.

中文翻译：

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通过半溶解壳聚糖模板制备具有高比表面积的大孔纤维素微球用于蛋白质分离


大孔结构与高比表面积之间的冲突强烈限制了色谱介质的动态吸附性能。在这里，使用半溶解的壳聚糖作为双功能模板制备大孔和高比表面积纤维素微球（PCM），然后用二乙氨基乙基（DEAE）作为阴离子交换剂进行功能化以用于蛋白质分离。双功能模板策略利用溶解的壳聚糖纳米纤维作为介孔/微孔模板，利用未溶解的壳聚糖颗粒作为大孔模板。 DEAE-PCM 的大孔（平均尺寸：0.4–1.2 μm）可有效降低传质阻力，实现快速吸收动力学。高比表面积 (272.34 m/g) 提供了足够的配体位点，以确保卓越的吸附能力。因此，具有宏观/介观/微孔结构的壳聚糖双功能模板相变材料是高性能色谱介质的有希望的候选者。