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Dietary manganese supplementation decreases hepatic lipid deposition by regulating gene expression and enzyme activity involved in lipid metabolism in the liver of broilers
Journal of Animal Science ( IF 2.7 ) Pub Date : 2024-08-16 , DOI: 10.1093/jas/skae235
Ke Yang 1, 2, 3 , Xiaoyan Cui 1 , Yangyang Hu 1 , Xinyu Feng 1 , Wenpeng Chen 1 , Weiyun Zhang 1 , Liyang Zhang 4 , Sufen Li 2, 3 , Yun Hu 1 , Tingting Li 1 , Shengchen Wang 1 , Xugang Luo 1
Journal of Animal Science ( IF 2.7 ) Pub Date : 2024-08-16 , DOI: 10.1093/jas/skae235
Ke Yang 1, 2, 3 , Xiaoyan Cui 1 , Yangyang Hu 1 , Xinyu Feng 1 , Wenpeng Chen 1 , Weiyun Zhang 1 , Liyang Zhang 4 , Sufen Li 2, 3 , Yun Hu 1 , Tingting Li 1 , Shengchen Wang 1 , Xugang Luo 1
Affiliation
This study aimed to characterize the effects of different dietary forms of supplemental manganese (Mn) on hepatic lipid deposition, gene expression, and enzyme activity in liver fat metabolism in 42-d-old broiler chickens. In total 420 one-day-old Arbor Acres (AA) broilers (rooster:hen = 1:1) were assigned randomly based on body weight and sex to 1 of 6 treatments (10 replicate cages per treatment and 7 broilers per replicate cage) in a completely randomized design using a 2 (sex) × 3 (diet) factorial arrangement. The 3 diets were basal control diets without Mn supplementation and basal diets supplemented with either Mn sulfate or Mn proteinate. No sex × diet interactions were observed in any of the measured indexes; thus, the effect of diet alone was presented in this study. Dietary Mn supplementation increased Mn content in the plasma and liver, adipose triglyceride lipase (ATGL) activity, and ATGL mRNA and its protein expression in the liver by 5.3% to 24.0% (P < 0.05), but reduced plasma triglyceride (TG), total cholesterol, and low-density lipoprotein (LDL-C) levels, liver TG content, fatty acid synthase (FAS) and malic enzyme (ME) activities, mRNA expression of sterol-regulatory element-binding protein 1 (SREBP1), FAS, stearoyl-coA desaturase (SCD), and ME, as well as the protein expression of SREBP1 and SCD in the liver by 5.5% to 22.8% (P < 0.05). No differences were observed between the 2 Mn sources in all of the determined parameters. Therefore, it was concluded that dietary Mn supplementation, regardless of Mn source, decreased hepatic lipid accumulation in broilers by inhibiting SREBP1 and SCD expression, FAS and ME activities, and enhancing ATGL expression and activity.
中文翻译:
日粮锰补充剂通过调节肉鸡肝脏脂质代谢中参与的基因表达和酶活性来减少肝脏脂质沉积
本研究旨在表征不同日粮形式的锰 (Mn) 补充剂对 42 d 肉鸡肝脏脂质沉积、基因表达和肝脂肪代谢酶活性的影响。总共 420 只一日龄 Arbor Acres (AA) 肉鸡(公鸡:母鸡 = 1:1)根据体重和性别随机分配到 6 个处理中的 1 个(每个处理 10 个重复笼和每个重复笼 7 个肉鸡),采用 2(性别)× 3(饮食)因子安排。这 3 种日粮是不添加 Mn 的基础对照日粮和添加硫酸锰或锰蛋白的基础日粮。在任何测量指标中均未观察到性别×饮食相互作用;因此,本研究提出了单独饮食的效果。膳食补充 Mn 使血浆和肝脏中 Mn 含量、脂肪甘油三酯脂肪酶 (ATGL) 活性以及肝脏中 ATGL mRNA 及其蛋白表达增加 5.3% 至 24.0% (P < 0.05),但降低血浆甘油三酯 (TG) 、总胆固醇和低密度脂蛋白 (LDL-C) 水平、肝脏 TG 含量、脂肪酸合成酶 (FAS) 和苹果酸酶 (ME) 活性, 甾醇调节元件结合蛋白 1 (SREBP1) 、 FAS 、硬脂酰辅酶 A 去饱和酶 (SCD) 和 ME 的 mRNA 表达,以及肝脏中 SREBP1 和 SCD 的蛋白表达 5.5% 至 22.8% (P < 0.05)。在所有确定的参数中,2 Mn 源之间未观察到差异。因此,得出结论,无论 Mn 来源如何,日粮 Mn 补充剂通过抑制 SREBP1 和 SCD 表达、FAS 和 ME 活性以及增强 ATGL 表达和活性来减少肉鸡肝脏脂质积累。
更新日期:2024-08-16
中文翻译:
日粮锰补充剂通过调节肉鸡肝脏脂质代谢中参与的基因表达和酶活性来减少肝脏脂质沉积
本研究旨在表征不同日粮形式的锰 (Mn) 补充剂对 42 d 肉鸡肝脏脂质沉积、基因表达和肝脂肪代谢酶活性的影响。总共 420 只一日龄 Arbor Acres (AA) 肉鸡(公鸡:母鸡 = 1:1)根据体重和性别随机分配到 6 个处理中的 1 个(每个处理 10 个重复笼和每个重复笼 7 个肉鸡),采用 2(性别)× 3(饮食)因子安排。这 3 种日粮是不添加 Mn 的基础对照日粮和添加硫酸锰或锰蛋白的基础日粮。在任何测量指标中均未观察到性别×饮食相互作用;因此,本研究提出了单独饮食的效果。膳食补充 Mn 使血浆和肝脏中 Mn 含量、脂肪甘油三酯脂肪酶 (ATGL) 活性以及肝脏中 ATGL mRNA 及其蛋白表达增加 5.3% 至 24.0% (P < 0.05),但降低血浆甘油三酯 (TG) 、总胆固醇和低密度脂蛋白 (LDL-C) 水平、肝脏 TG 含量、脂肪酸合成酶 (FAS) 和苹果酸酶 (ME) 活性, 甾醇调节元件结合蛋白 1 (SREBP1) 、 FAS 、硬脂酰辅酶 A 去饱和酶 (SCD) 和 ME 的 mRNA 表达,以及肝脏中 SREBP1 和 SCD 的蛋白表达 5.5% 至 22.8% (P < 0.05)。在所有确定的参数中,2 Mn 源之间未观察到差异。因此,得出结论,无论 Mn 来源如何,日粮 Mn 补充剂通过抑制 SREBP1 和 SCD 表达、FAS 和 ME 活性以及增强 ATGL 表达和活性来减少肉鸡肝脏脂质积累。