This study aimed to characterize the effects of different dietary forms of supplemental manganese (Mn) on hepatic lipid deposition, gene expression, and enzyme activity in liver fat metabolism in 42-day-old broiler chickens. In total 420 one day-old Arbor Acres (AA) broilers (rooster: hen = 1:1) were assigned randomly based on body weight and sex to one of six treatments (ten replicate cages per treatment and seven broilers per replicate cage) in a completely randomized design using a 2 (sex) × 3 (diet) factorial arrangement. The three diets were basal control diets without Mn supplementation and basal diets supplemented with either Mn sulfate or Mn proteinate. No sex × diet interactions were observed in any of the measured indexes; thus, the effect of diet alone was presented in this study. Dietary Mn supplementation increased Mn content in the plasma and liver, adipose triglyceride lipase (ATGL) activity, and ATGL mRNA and its protein expression in the liver by 5.3%–24.0% (P < 0.05), but reduced plasma triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL-C) levels, liver TG content, fatty acid synthase (FAS) and malic enzyme (ME) activities, mRNA expression of sterol regulatory element-binding protein 1 (SREBP1), FAS, stearoyl-coA desaturase (SCD), and ME, as well as the protein expression of SREBP1 and SCD in the liver by 5.5%–22.8% (P < 0.05). No differences were observed between the two Mn sources in all of the determined parameters. Therefore, it was concluded that dietary Mn supplementation, regardless of Mn source, decreased hepatic lipid accumulation in broilers by inhibiting SREBP1 and SCD expression, FAS and ME activities, and enhancing ATGL expression and activity.

中文翻译：

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日粮补充锰通过调节肉鸡肝脏脂质代谢相关基因表达和酶活性来减少肝脏脂质沉积


本研究旨在表征不同日粮形式补充锰 (Mn) 对 42 日龄肉鸡肝脏脂质沉积、基因表达和肝脏脂肪代谢酶活性的影响。总共 420 只 1 日龄 Arbor Acres (AA) 肉鸡（公鸡：母鸡 = 1:1）根据体重和性别随机分配至 6 个处理之一（每个处理 10 个重复笼，每个重复笼 7 只肉鸡）使用 2（性别）× 3（饮食）因子排列的完全随机设计。这三种日粮是未补充锰的基础对照日粮和补充硫酸锰或蛋白盐的基础日粮。任何测量指标均未观察到性别×饮食相互作用；因此，本研究仅介绍了饮食的影响。膳食补充锰使血浆和肝脏中的锰含量、肝脏中脂肪甘油三酯脂肪酶（ATGL）活性和ATGL mRNA及其蛋白表达增加5.3%–24.0%（P<<0.05），但降低了血浆甘油三酯（TG） 、总胆固醇 (TC) 和低密度脂蛋白 (LDL-C) 水平、肝脏 TG 含量、脂肪酸合酶 (FAS) 和苹果酸酶 (ME) 活性、甾醇调节元件结合蛋白 1 (SREBP1) 的 mRNA 表达、FAS、硬脂酰辅酶A去饱和酶（SCD）和ME，以及肝脏中SREBP1和SCD的蛋白表达量增加了5.5%–22.8%（P< 0.05）。在所有确定的参数中，两种锰源之间没有观察到差异。因此，得出的结论是，无论锰来源如何，日粮中添加锰都可以通过抑制SREBP1和SCD表达、FAS和ME活性以及增强ATGL表达和活性来减少肉鸡肝脏脂质积累。