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Unveiling Cellular Microenvironments with a Near-Infrared Fluorescent Sensor: A Dual-Edge Tool for Cancer Detection and Drug Screening
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-08-15 , DOI: 10.1021/acs.analchem.4c03155 Bin Li 1 , Chenchen Bian 1 , Lei Yang 2 , Yanxi Zhu 3 , Zhanxian Li 1 , Mingming Yu 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-08-15 , DOI: 10.1021/acs.analchem.4c03155 Bin Li 1 , Chenchen Bian 1 , Lei Yang 2 , Yanxi Zhu 3 , Zhanxian Li 1 , Mingming Yu 1
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Mitochondria and lysosomes are pivotal intracellular organelles, and the injury or dysfunction of these organelles can trigger a range of pathological processes. Early diagnosis and treatment of cancer are of paramount importance due to cancer’s status as a leading health threat. This study introduces a novel fluorescent probe, BDHV, for detecting mitochondrial and lysosomal viscosity and pH abnormalities in tumors, facilitating early cancer detection and screening of anticancer drugs. Under acidic conditions, the red fluorescence of the probe gradually increases with increasing viscosity. Conversely, in alkaline environments, an increase in viscosity leads to a decrease in green fluorescence and an increase in red fluorescence. The inclusion of a benzothiazole group endows BDHV with strong dual-targeting capability for mitochondria and lysosomes and without being affected by the mitochondrial membrane potential. Most notably, BDHV has potential applications for early cancer diagnosis and in effectively assessing the efficacy of various anticancer drugs.
中文翻译：
用近红外荧光传感器揭示细胞微环境：癌症检测和药物筛选的双刃工具
线粒体和溶酶体是关键的细胞内细胞器，这些细胞器的损伤或功能障碍可以引发一系列病理过程。由于癌症是主要的健康威胁，因此癌症的早期诊断和治疗至关重要。本研究引入了一种新型荧光探针BDHV ，用于检测肿瘤中线粒体和溶酶体粘度和pH值异常，促进早期癌症检测和抗癌药物筛选。在酸性条件下，探针的红色荧光随着粘度的增加而逐渐增强。相反，在碱性环境中，粘度增加导致绿色荧光减少，红色荧光增加。苯并噻唑基团的包含使BDHV具有强大的线粒体和溶酶体双靶向能力，且不受线粒体膜电位的影响。最值得注意的是， BDHV在早期癌症诊断和有效评估各种抗癌药物的疗效方面具有潜在的应用。
更新日期：2024-08-15
中文翻译：
用近红外荧光传感器揭示细胞微环境：癌症检测和药物筛选的双刃工具
线粒体和溶酶体是关键的细胞内细胞器，这些细胞器的损伤或功能障碍可以引发一系列病理过程。由于癌症是主要的健康威胁，因此癌症的早期诊断和治疗至关重要。本研究引入了一种新型荧光探针BDHV ，用于检测肿瘤中线粒体和溶酶体粘度和pH值异常，促进早期癌症检测和抗癌药物筛选。在酸性条件下，探针的红色荧光随着粘度的增加而逐渐增强。相反，在碱性环境中，粘度增加导致绿色荧光减少，红色荧光增加。苯并噻唑基团的包含使BDHV具有强大的线粒体和溶酶体双靶向能力，且不受线粒体膜电位的影响。最值得注意的是， BDHV在早期癌症诊断和有效评估各种抗癌药物的疗效方面具有潜在的应用。
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