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Highly sensitive CD40L protein discrimination via label-free fiber sensing technologies
Analyst ( IF 3.6 ) Pub Date : 2024-08-16 , DOI: 10.1039/d4an00867g Mingxuan Hu 1 , Xuying Li 2 , Yansong Li 3 , Ruiduo Wang 1, 4 , Tongyuan Kang 1 , Fuxing Xu 5 , Jintao Bai 1 , Man Jiang 1 , Yaomin Zhu 3
Analyst ( IF 3.6 ) Pub Date : 2024-08-16 , DOI: 10.1039/d4an00867g Mingxuan Hu 1 , Xuying Li 2 , Yansong Li 3 , Ruiduo Wang 1, 4 , Tongyuan Kang 1 , Fuxing Xu 5 , Jintao Bai 1 , Man Jiang 1 , Yaomin Zhu 3
Affiliation
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The recombinant Cluster of Differentiation 40 Ligand (CD40L) can be expressed in various cells and is closely related to various types of cancer. This association underscores the critical need for expedited and precise measurement of CD40L levels in clinical fluid specimens. A novel optical fiber biosensor has been devised, employing single-mode fibers that are sandwiched around a coreless fiber, with the diameter refined by etching with hydrogen fluoride. This innovative configuration allows for light transmission through the evanescent field, thereby enhancing the sensor's sensitivity to changes in the surrounding refractive index. Employing chemical binding techniques, CD40 was securely immobilized onto the fiber's surface, facilitating the detection of CD40L. The sensor exhibited a sensitivity of 1.126 nm (μg mL−1)−1 and a detection limit of 0.68 nM. Furthermore, the sensor's specificity for CD40L was validated using authentic clinical serum samples spiked with artificial analytes. In addition, the specificity of CD40L of the proposed sensor was proved using natural clinical serum samples with added artificial analyte, assisted by the ELISA method, and the results ideally conformed with the detection of standard samples. With the aid of the ELISA method, the outcomes were found to be in excellent agreement with those from standard sample detection. Consequently, the findings indicate that this sensor provides a specific, label-free, and highly sensitive method for CD40L detection, showcasing its significant potential for applications in molecular biology research.
中文翻译：
通过无标记光纤传感技术实现高度灵敏的 CD40L 蛋白辨别
重组分化簇40配体（CD40L）可在多种细胞中表达，与多种癌症密切相关。这种关联强调了快速、精确测量临床液体样本中 CD40L 水平的迫切需要。人们设计了一种新型光纤生物传感器，采用夹在无芯光纤周围的单模光纤，并通过氟化氢蚀刻来细化直径。这种创新配置允许光通过倏逝场传输，从而增强传感器对周围折射率变化的灵敏度。采用化学结合技术，CD40 被牢固地固定在纤维表面，有利于 CD40L 的检测。该传感器表现出1.126 nm (μg mL -1 ) -1的灵敏度和0.68 nM的检测限。此外，使用掺有人工分析物的真实临床血清样品验证了传感器对 CD40L 的特异性。此外，利用添加人工分析物的天然临床血清样品，并辅以ELISA方法，证明了该传感器CD40L的特异性，结果与标准样品的检测结果非常吻合。借助 ELISA 方法，发现结果与标准样品检测结果非常一致。因此，研究结果表明，该传感器为 CD40L 检测提供了一种特异性、无标记且高灵敏度的方法，展示了其在分子生物学研究中的巨大应用潜力。
更新日期：2024-08-16
中文翻译：
通过无标记光纤传感技术实现高度灵敏的 CD40L 蛋白辨别
重组分化簇40配体（CD40L）可在多种细胞中表达，与多种癌症密切相关。这种关联强调了快速、精确测量临床液体样本中 CD40L 水平的迫切需要。人们设计了一种新型光纤生物传感器，采用夹在无芯光纤周围的单模光纤，并通过氟化氢蚀刻来细化直径。这种创新配置允许光通过倏逝场传输，从而增强传感器对周围折射率变化的灵敏度。采用化学结合技术，CD40 被牢固地固定在纤维表面，有利于 CD40L 的检测。该传感器表现出1.126 nm (μg mL -1 ) -1的灵敏度和0.68 nM的检测限。此外，使用掺有人工分析物的真实临床血清样品验证了传感器对 CD40L 的特异性。此外，利用添加人工分析物的天然临床血清样品，并辅以ELISA方法，证明了该传感器CD40L的特异性，结果与标准样品的检测结果非常吻合。借助 ELISA 方法，发现结果与标准样品检测结果非常一致。因此，研究结果表明，该传感器为 CD40L 检测提供了一种特异性、无标记且高灵敏度的方法，展示了其在分子生物学研究中的巨大应用潜力。
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