Quantitative Assessment of Collagen Remodeling during a Murine Pregnancy,ACS Photonics

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Quantitative Assessment of Collagen Remodeling during a Murine Pregnancy
ACS Photonics ( IF 6.5 ) Pub Date : 2024-08-14 , DOI: 10.1021/acsphotonics.4c00337
Jessica C. Ramella-Roman ₁ , Mala Mahendroo ₂ , Clothilde Raoux ₃ , Gaël Latour _{3,

4} , Marie-Claire Schanne-Klein ₃

Affiliation

Uterine cervical remodeling is a fundamental feature of pregnancy, facilitating the delivery of the fetus through the cervical canal. Yet, we still know very little about this process due to the lack of methodologies that can quantitatively and unequivocally pinpoint the changes the cervix undergoes during pregnancy. We utilize polarization-resolved second harmonic generation to visualize the alterations the cervix extracellular matrix, specifically collagen, undergoes during pregnancy with exquisite resolution. This technique provides images of the collagen orientation at the pixel level (0.4 μm) over the entire murine cervical section. They show tight and ordered packing of collagen fibers around the os at the early stage of pregnancy and their disruption at the later stages. Furthermore, we utilize a straightforward statistical analysis to demonstrate the loss of order in the tissue, consistent with the loss of mechanical properties associated with this process. This work provides a deeper understanding of the parturition process and could support research into the cause of pathological or premature birth.

中文翻译：

小鼠妊娠期间胶原重塑的定量评估

宫颈重塑是妊娠的一个基本特征，有助于胎儿通过宫颈管分娩。然而，由于缺乏能够定量、明确地确定怀孕期间子宫颈所经历的变化的方法，我们对这一过程仍然知之甚少。我们利用偏振分辨二次谐波产生以精细的分辨率可视化妊娠期间子宫颈细胞外基质（特别是胶原蛋白）发生的变化。该技术提供了整个小鼠宫颈切片的像素级 (0.4 μm) 胶原蛋白取向图像。它们显示出妊娠早期口腔周围胶原纤维紧密有序的堆积，以及妊娠后期的破坏。此外，我们利用简单的统计分析来证明组织中秩序的丧失，这与与此过程相关的机械性能的损失一致。这项工作提供了对分娩过程的更深入的了解，并可以支持对病理性或早产原因的研究。

更新日期：2024-08-14

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