An aromatic ring-containing compound with a wide range of biological activities, 9-methylacridine (AD-9-Me) is a precursor for the synthesis of various drugs. However, its photoactivation properties and mechanism of damage as a photo activator against Aedes aegypti are unknown. The toxic effects of AD-9-Me on Aedes aegypti mosquitoes were determined under light and non-light conditions. The results showed that the toxicity of AD-9-Me to mosquito larvae was significantly higher than that of the dark treatment after 24 h of light exposure; AD-9-Me was mainly distributed in the midgut of larvae, after 24 h of treatment, it can cause an increase in calcium ion concentration, reactive oxygen species (ROS) eruption and ROS accumulation by blocking the ROS elimination pathway in midgut cells. This in turn caused an increase in protein carbonyl and malondialdehyde (MDA) content, a decrease in mitochondrial membrane potential (MMP), a disruption of the barrier function of midgut tissues, a significant decrease in midgut weight and chitin content, which induced the up-regulation of AeDronc, AeCaspase8 and AeCaspase7 genes, leading to apoptotic cell death. In this study, we confirmed that AD-9-Me has photoactivation activity and mainly acts on the midgut of mosquito larvae, which can generate a large amount of ROS in the cells of the midgut and induce apoptosis to occur, resulting in the disruption of the function of the tissues of mosquito larvae, accelerating the death and delaying the development of the mosquito larvae.

中文翻译：

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光激活的 9-甲基吖啶通过靶向中肠细胞线粒体途径中 ROS 介导的细胞凋亡来破坏埃及伊蚊幼虫的中肠组织。


9-甲基吖啶（AD-9-Me）是一种具有广泛生物活性的含芳香环化合物，是合成多种药物的前体。然而，其作为抗埃及伊蚊的光活化剂的光活化特性和损伤机制尚不清楚。在光照和非光照条件下测定了 AD-9-Me 对埃及伊蚊的毒性作用。结果表明，光照24 h后，AD-9-Me对蚊幼虫的毒性明显高于暗处理； AD-9-Me主要分布于幼虫中肠，处理24 h后，通过阻断中肠细胞ROS消除途径，导致钙离子浓度增加、活性氧（ROS）喷发和ROS积累。这反过来导致蛋白质羰基和丙二醛（MDA）含量增加，线粒体膜电位（MMP）降低，中肠组织屏障功能破坏，中肠重量和几丁质含量显着下降，从而导致-AeDronc、AeCaspase8 和 AeCaspase7 基因的调节，导致细胞凋亡。在本研究中，我们证实AD-9-Me具有光激活活性，主要作用于蚊子幼虫的中肠，可以在中肠细胞中产生大量ROS并诱导细胞凋亡发生，导致蚊子幼虫的细胞凋亡被破坏。影响蚊子幼虫组织的功能，加速蚊子幼虫的死亡并延缓其发育。