Both the phyllosphere and rhizosphere are inhabited by different kinds of microorganisms that are closely related to plant growth and health. However, it is not clear whether disease-resistant cultivars shape the microbiome to facilitate disease resistance. In this study, significant differences were found in the aboveground and belowground bacterial communities of disease-resistant and disease-susceptible cultivars grown in the same kiwifruit orchard. The phyllosphere of the resistant cultivar ‘Wanjin’ showed greater enrichment of Pseudomonas spp. and Sphingomonas spp. than the susceptible cultivar ‘Donghong’. The rhizosphere microbes of ‘Wanjin’ were less affected by field location, with significantly greater bacterial abundance than those of ‘Donghong’ and more bacteria with potential biocontrol properties. Pseudomonas syringae pv. actinidiae (Psa) infection significantly affected the microbiome of the phyllosphere of kiwifruit plants, especially that of ‘Donghong’. Resistant and susceptible kiwifruit cultivars exhibit distinct beneficial microbial recruitment strategies under Psa challenge. The phyllosphere of ‘Donghong’ in Jinzhai was enriched with Sphingomonas spp. and Pantoea spp. under Psa infection, while the rhizosphere of ‘Wanjin’ was enriched with Sphingomonas spp. and Novosphingobium spp. We further identified five key biomarkers within the microbial community associated with Psa infection. Inoculation experiments showed that Lysobacter sp. R34, Stenotrophomonas sp. R31, Pseudomonas sp. R10 and RS54, which were isolated from belowground compartments of ‘Wanjin’, could positively affect plant performance under Psa challenge. The combination use of Pseudomonas sp. R10 and Stenotrophomonas sp. R31 significantly improve the management of kiwifruit canker. Our findings provided novel insights into soil–microbe–plant interactions and the role of microbes in plant disease resistance and susceptibility.

中文翻译：

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基因型相关核心细菌增强了宿主对猕猴桃细菌性溃疡病的抵抗力


叶际和根际都栖息着与植物生长和健康密切相关的不同种类的微生物。然而，目前尚不清楚抗病品种是否塑造微生物组以促进抗病性。在这项研究中，在同一猕猴桃园中生长的抗病和易病品种的地上和地下细菌群落中发现了显著差异。抗性品种 '万金' 的叶际显示假单胞菌属和鞘氨醇单胞菌属的富集程度高于感病品种 '东红'。'万锦'根际微生物受田间位置的影响较小，细菌丰度显著高于'东洪'，具有潜在生防特性的细菌较多。丁香假单胞菌 pv.猕猴桃科 （psa） 感染显著影响猕猴桃植株叶状层的微生物组，尤其是'东红'的微生物组。抗性和易感猕猴桃品种在 Psa 攻击下表现出独特的有益微生物募集策略。金寨'东洪'的叶际富含Psa感染下的鞘氨醇单胞菌属和Pantoea属，而'万金'的根际富含鞘氨醇单胞菌属和新鞘氨醇属。我们进一步确定了与 Psa 感染相关的微生物群落中的五个关键生物标志物。接种实验表明，溶血单胞菌属 R34、狭窄单胞菌属 R31、假单胞菌属。从'万金'地下隔室中分离出的R10和RS54在Psa胁迫下对植物性能产生积极影响。Pseudomonas sp. 的组合用途。R10 和 Stenotrophomonas sp.R31 显着改善猕猴桃溃疡病的管理。 我们的研究结果为土壤-微生物-植物相互作用以及微生物在植物抗病性和易感性中的作用提供了新的见解。