Both the phyllosphere and rhizosphere are inhabited by different kinds of microorganisms that are closely related to plant growth and health. However, it is not clear whether disease-resistant cultivars shape the microbiome to facilitate disease resistance. In this study, significant differences were found in the aboveground and belowground bacterial communities of disease-resistant and disease-susceptible cultivars grown in the same kiwifruit orchard. The phyllosphere of the resistant cultivar “Wanjin” was more strongly affected by geographic factors and showed greater enrichment of Pseudomonas spp. and Sphingomonas spp. than the susceptible cultivar “Donghong”. The rhizosphere microbes of “Wanjin” were less affected by field location, with significantly greater bacterial abundance, than those of “Donghong”, and more bacteria with potential biocontrol properties. Pseudomonas syringae pv. actinidiae (Psa) infection significantly affected the microbiome of the phyllosphere of kiwifruit plants, especially that of “Donghong”. Resistant and susceptible kiwifruit cultivars exhibit distinct beneficial microbial recruitment strategies under Psa challenge. The phyllosphere of “Donghong” in Jinzhai was enriched with Sphingomonas spp. and Pantoea spp. under Psa infection, while the rhizosphere of “Wanjin” was enriched with Sphingomonas spp. and Novosphingobium spp. We further identified five key biomarkers within the microbial community associated with Psa infection. Detached-branch inoculation experiments showed that Lysobacter sp. R34, Stenotrophomonas sp. R31, Pseudomonas sp. R10 and RS54, which were isolated from the root endosphere or rhizosphere of “Wanjin”, could positively affect plant performance under Psa challenge. Our findings provided novel insights into soil–microbe–plant interactions and the role of microbes in plant disease resistance and susceptibility.

中文翻译：

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基因型相关核心细菌增强宿主对猕猴桃细菌性溃疡病的抵抗力


叶际和根际都栖息着与植物生长和健康密切相关的不同种类的微生物。然而，尚不清楚抗病品种是否会塑造微生物组以促进抗病性。本研究发现同一猕猴桃园内抗病品种和感病品种的地上和地下细菌群落存在显着差异。抗性品种“万金”的叶际受地理因素影响更强烈，假单胞菌富集程度更高。和鞘氨醇单胞菌属。高于感病品种“东红”。 “万金”根际微生物受田间位置影响较小，细菌丰度显着高于“东红”，且具有潜在生防作用的细菌较多。丁香假单胞菌 pv.猕猴桃（Psa）感染显着影响猕猴桃植物叶际微生物组，尤其是“东红”。抗性和易感猕猴桃品种在 Psa 挑战下表现出独特的有益微生物招募策略。金寨“东红”叶际富含鞘氨醇单胞菌。和泛菌属。在Psa感染下，而“万金”的根际富含鞘氨醇单胞菌属。和新鞘氨醇属。我们进一步确定了与 Psa 感染相关的微生物群落中的五个关键生物标志物。离枝接种实验表明溶菌属 sp。 R34，寡养单胞菌属。 R31，假单胞菌属。从“万金”根内圈或根际分离的 R10 和 RS54，可以在 Psa 挑战下对植物性能产生积极影响。 我们的研究结果为土壤-微生物-植物相互作用以及微生物在植物抗病性和易感性中的作用提供了新的见解。