Characterization of vaginal Lactobacillus in biologically relevant fluid using surface-enhanced Raman spectroscopy,Analyst

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Characterization of vaginal Lactobacillus in biologically relevant fluid using surface-enhanced Raman spectroscopy
Analyst ( IF 3.6 ) Pub Date : 2024-08-12 , DOI: 10.1039/d4an00854e
Anna S Rourke-Funderburg _{1,

2} , Anita Mahadevan-Jansen _{1,

2} , Andrea K Locke _{1,

2,

3}

Affiliation

The native vaginal microbiome plays a crucial role in maintaining vaginal health and disruption can have significant consequences for women during their lifetime. While the composition of the vaginal microbiome is important, current methods for monitoring this community are lacking. Clinically used techniques routinely rely on subjective analysis of vaginal fluid characteristics or time-consuming microorganism culturing. Surface-enhanced Raman spectroscopy (SERS) can aid in filling this gap in timely detection of alterations in the vaginal microbiome as it can discriminate between bacterial species in complex solutions including bacterial mixtures and biofluids. SERS has not previously been applied to study variations in vaginal Lactobacillus, the most common species found in the vaginal microbiome, in complex solutions. Herein, the SERS spectra of Lactobacillus crispatus (L. crispatus) and Lactobacillus iners (L. iners), two of the most common vaginal bacteria, was characterized at physiologically relevant concentrations. Subsequently, the ability of SERS to detect L. crispatus and L. iners in both pure mixtures and when mixed with a synthetic vaginal fluid mimicking solution was determined. In both pure and complex solutions, SERS coupled with partial least squares regression predicted the ratiometric bacterial content with less than 10% error and strong goodness of prediction (Q² > 0.9). This developed method highlights the applicability of SERS to predict the dominant Lactobacillus in the vaginal micro-environment toward the monitoring of this community.

中文翻译：

使用表面增强拉曼光谱表征生物相关液体中的阴道乳杆菌

原生阴道微生物组在维持阴道健康方面发挥着至关重要的作用，其破坏可能会对女性一生产生重大影响。虽然阴道微生物群的组成很重要，但目前缺乏监测该群落的方法。临床使用的技术通常依赖于对阴道液体特征的主观分析或耗时的微生物培养。表面增强拉曼光谱 (SERS) 可以帮助填补这一空白，及时检测阴道微生物组的变化，因为它可以区分复杂溶液（包括细菌混合物和生物液体）中的细菌种类。 SERS 此前尚未用于研究复杂溶液中阴道乳杆菌（阴道微生物组中最常见的菌种）的变化。在此，对两种最常见的阴道细菌卷曲乳杆菌（ L.crispatus ）和惰性乳杆菌（ L.iners ）的 SERS 光谱进行了生理相关浓度的表征。随后，确定了 SERS 在纯混合物中以及与合成阴道液模拟溶液混合时检测卷曲乳杆菌和惰性乳杆菌的能力。在纯溶液和复杂溶液中，SERS 与偏最小二乘回归相结合预测了比率细菌含量，误差小于 10%，预测精度很高 ( Q ² > 0.9)。这种开发的方法强调了 SERS 预测阴道微环境中主要乳酸菌的适用性，以监测该群落。

更新日期：2024-08-12

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