Ferroptosis has been recognized as a unique cell death modality driven by excessive lipid peroxidation and unbalanced cellular metabolism. In this study, we established a protein interaction landscape for ferroptosis pathways through proteomic analyses, and identified choline/ethanolamine phosphotransferase 1 (CEPT1) as a lysophosphatidylcholine acyltransferase 3 (LPCAT3)-interacting protein that regulates LPCAT3 protein stability. In contrast to its known role in promoting phospholipid synthesis, we showed that CEPT1 suppresses ferroptosis potentially by interacting with phospholipases and breaking down certain pro-ferroptotic polyunsaturated fatty acid (PUFA)-containing phospholipids. Together, our study reveals a previously unrecognized role of CEPT1 in suppressing ferroptosis.

中文翻译：

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铁死亡途径的蛋白质组学分析揭示了 CEPT1 在抑制铁死亡中的作用。


铁死亡被认为是一种独特的细胞死亡方式，由过度的脂质过氧化和不平衡的细胞代谢驱动。在这项研究中，我们通过蛋白质组学分析建立了铁死亡途径的蛋白质相互作用图谱，并鉴定出胆碱/乙醇胺磷酸转移酶 1 (CEPT1) 是一种调节 LPCAT3 蛋白质稳定性的溶血磷脂酰胆碱酰基转移酶 3 (LPCAT3) 相互作用蛋白。与其已知的促进磷脂合成的作用相反，我们发现CEPT1可能通过与磷脂酶相互作用并分解某些促铁死亡的含多不饱和脂肪酸（PUFA）的磷脂来抑制铁死亡。总之，我们的研究揭示了 CEPT1 在抑制铁死亡方面以前未被认识到的作用。