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Centimetre scale functional dispersal limitation of freshwater copiotrophs
Environmental Microbiology ( IF 4.3 ) Pub Date : 2024-08-12 , DOI: 10.1111/1462-2920.16682 Marie Dannenmann 1, 2 , Alizée Le Moigne 1, 3 , Cyrill Hofer 1 , Jakob Pernthaler 1
Environmental Microbiology ( IF 4.3 ) Pub Date : 2024-08-12 , DOI: 10.1111/1462-2920.16682 Marie Dannenmann 1, 2 , Alizée Le Moigne 1, 3 , Cyrill Hofer 1 , Jakob Pernthaler 1
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The freshwater microbiome harbours numerous copiotrophic bacteria that rapidly respond to elevated substrate concentrations. We hypothesized that their high centimetre‐scale beta diversity in lake water translates into pronounced metabolic variability, and that a large fraction of microbial ‘metabolic potential’ originates from point sources such as fragile organic aggregates. Three experiments were conducted in pre‐alpine Lake Zurich over the course of a harmful cyanobacterial bloom: Spatially explicit 9 ml ‘syringe’ samples were collected in situ at centimetre distances along with equally sized ‘mixed’ samples drawn from pre‐homogenized lake water and incubated in BIOLOG EcoPlate substrate arrays. Fewer compounds promoted bacterial growth in the syringe than in the mixed samples, in particular during the pre‐ and late bloom periods. Community analysis of enrichments on three frequently utilized substrates revealed both pronounced heterogeneity and functional redundancy. Bacterial consortia had higher richness in mixed than in syringe samples and differed in composition. Members of the Enterobacter cloacae complex dominated the EcoPlate assemblages during the mid‐bloom period irrespective of treatment or substrate. We conclude that small‐scale functional dispersal limitation among free‐living copiotrophs in lake water reduces local biotransformation potential, and that lacustrine blooms of harmful cyanobacteria can be environmental reservoirs for metabolically versatile potential pathogens.
中文翻译：
淡水富营养生物的厘米级功能性扩散限制
淡水微生物组含有大量富营养细菌，它们对升高的底物浓度迅速做出反应。我们假设湖水中的高厘米级β多样性转化为显着的代谢变异性，并且微生物“代谢潜力”的很大一部分源自点源，例如脆弱的有机聚集体。在有害的蓝藻水华发生过程中，在高山前的苏黎世湖进行了三项实验：在厘米距离处原位采集了空间明确的 9 毫升“注射器”样本，以及从预均质化的湖水中抽取的同等大小的“混合”样本，在 BIOLOG EcoPlate 基质阵列中孵育。与混合样品相比，注射器中促进细菌生长的化合物更少，特别是在开花前期和后期。对三种常用底物富集的群落分析揭示了明显的异质性和功能冗余。混合样品中的细菌群落比注射器样品中的细菌群落丰富度更高，并且组成也不同。成员阴沟肠杆菌无论处理或基质如何，复合体在开花中期的 EcoPlate 组合中占主导地位。我们得出的结论是，湖水中自由生活的富营养生物的小规模功能扩散限制降低了局部生物转化潜力，并且有害蓝细菌的湖水华可能是代谢多功能潜在病原体的环境库。
更新日期：2024-08-12
中文翻译：
淡水富营养生物的厘米级功能性扩散限制
淡水微生物组含有大量富营养细菌，它们对升高的底物浓度迅速做出反应。我们假设湖水中的高厘米级β多样性转化为显着的代谢变异性，并且微生物“代谢潜力”的很大一部分源自点源，例如脆弱的有机聚集体。在有害的蓝藻水华发生过程中，在高山前的苏黎世湖进行了三项实验：在厘米距离处原位采集了空间明确的 9 毫升“注射器”样本，以及从预均质化的湖水中抽取的同等大小的“混合”样本，在 BIOLOG EcoPlate 基质阵列中孵育。与混合样品相比，注射器中促进细菌生长的化合物更少，特别是在开花前期和后期。对三种常用底物富集的群落分析揭示了明显的异质性和功能冗余。混合样品中的细菌群落比注射器样品中的细菌群落丰富度更高，并且组成也不同。成员阴沟肠杆菌无论处理或基质如何，复合体在开花中期的 EcoPlate 组合中占主导地位。我们得出的结论是，湖水中自由生活的富营养生物的小规模功能扩散限制降低了局部生物转化潜力，并且有害蓝细菌的湖水华可能是代谢多功能潜在病原体的环境库。
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