This work aimed to obtain extracts from leaves through ultrasonic pretreatment in supercritical CO extraction (UAE+SFE). A central composite design was used to evaluate the influence of temperature (36–64 °C), pressure (8–20 MPa), and cosolvent (0–20 %w) in terms of overall yield and chemical composition. Morphology using scanning electron micrograph (SEM), total phenolic content, content of total flavonoid, antioxidant (DPPH and ABTS), and antibacterial activities were evaluated. UAE+SFE showed a more notable overall yield, with 6.97 %. In the SEM, the sample treated with UAE+SFE significantly impacted tissue structures, improving the selectivity of SFE regarding linolenic acid, leading to a maximum composition value of 62.5 % area according to GC-MS. Furthermore, the UAE+SFE extract exhibited strong antimicrobial activity compared to the extract obtained by the SFE technique. Based on the pioneering results, the bioactives obtained are promising and interesting for application in the cosmetic, pharmaceutical, and food industries.

中文翻译：

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超声波预处理联合超临界CO2萃取木香叶提取物


本工作旨在通过超临界 CO 萃取 (UAE+SFE) 中的超声波预处理获得叶子提取物。采用中心复合设计来评估温度（36-64°C）、压力（8-20 MPa）和助溶剂（0-20%w）对总产率和化学成分的影响。使用扫描电子显微照片（SEM）评估形态、总酚含量、总黄酮含量、抗氧化剂（DPPH和ABTS）和抗菌活性。 UAE+SFE 的整体收益率更为显着，为 6.97%。在 SEM 中，经 UAE+SFE 处理的样品显着影响了组织结构，提高了 SFE 对亚麻酸的选择性，根据 GC-MS 得出的最大成分值为 62.5% 面积。此外，与通过 SFE 技术获得的提取物相比，UAE+SFE 提取物表现出更强的抗菌活性。基于这些开创性的成果，所获得的生物活性物质在化妆品、制药和食品行业的应用前景广阔且令人感兴趣。