is an invasive plant containing Sulfated Flavonoids (SFs) with therapeutic potential. The extraction of these compounds would add value to the biomass generated during weeding. This work reports the optimization of the pretreatment of leaves with supercritical CO (P-SC-CO) prior extraction of these compounds using subcritical water extraction. The combined effect of different levels of the three factors on P-SC-CO, Time, Pressure and Depressurization rate, was investigated with Response Surface Methodology. The optimal pretreatment conditions were as follows: Time = 30 min, Pressure = 30 MPa and Depressurization rate = 2 kg CO/h. Under these conditions, 72.45 ± 0.99 mg of totals SFs/100 g of leaves was recovered, practically doubling total SFs extraction yield in the absence of pretreatment. In addition, the structural damage caused by P-SC-CO on the leaves was detected using Scanning Electron Microscopy.

中文翻译：

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亚临界水提取硫酸化黄酮前超临界二氧化碳预处理黄芪叶的优化


是一种入侵植物，含有具有治疗潜力的硫酸化黄酮类化合物 (SF)。这些化合物的提取将为除草过程中产生的生物量增加价值。这项工作报告了在使用亚临界水萃取提取这些化合物之前用超临界 CO (P-SC-CO) 对叶子进行预处理的优化。采用响应面法研究了时间、压力和降压速率三个因素的不同水平对 P-SC-CO 的综合影响。最佳预处理条件为：时间=30 min，压力=30 MPa，降压速率=2 kg CO/h。在这些条件下，每 100 克叶子回收了 72.45 ± 0.99 毫克总 SF，在没有预处理的情况下，总 SF 提取率几乎翻了一番。此外，使用扫描电子显微镜检测了 P-SC-CO 对叶片造成的结构损伤。