Plants use various secondary chemicals in their chemical defense against herbivores. While botanical insecticides are crucial for reducing the reliance on chemical pesticides, the development of plant-derived insecticides remains limited. In this study, we fed Henosepilachna vigintioctopunctata with three different host plants (Solanum nigrum, Solanum tuberosum, and Physalis floridana) and observed that feeding on P. floridana led to changes in the body size and a significantly high mortality rate. Through transcriptome analysis, it was found that the trehalose metabolism pathway of H. vigintioctopunctata changed significantly under different host feeding conditions, especially since the expression level of the trehalase gene was extremely different. We subsequently identified eight transcripts of HvTREs and analyzed their evolution and structure. Among them, significant differences are observed in the relative expression levels of HvTRE1-5 in H. vigintioctopunctata after the fourth instar and were affected by different plant diets. Compared with the natural host S. nigrum, the larvae that fed on P. floridana significantly reduced the contents of trehalose, glucose and glycogen and significantly affected the trehalase activity. Knockdown of HvTRE1-5 by RNAi increased mortality at the H. vigintioctopunctata prepupation stage, suggesting that HvTRE1-5 is important for H. vigintioctopunctata pupation. This study provides new insights into developing of green control methods for H. vigintioctopunctata and offers a valuable example for understanding the interaction between host plants and herbivorous insects.

植物使用各种次级化学物质来对食草动物进行化学防御。虽然植物杀虫剂对于减少对化学杀虫剂的依赖至关重要，但植物源杀虫剂的开发仍然有限。在这项研究中，我们用三种不同的寄主植物（龙葵、马铃薯和酸浆）喂养Henosepilachna vigintioctopunctata ，并观察到以P.floridana为食会导致体型变化和显着高的死亡率。通过转录组分析发现，不同寄主摄食条件下， H. vigintioctopunctata的海藻糖代谢途径发生显着变化，尤其是海藻糖酶基因的表达水平差异极大。我们随后鉴定了HvTRE的八个转录本并分析了它们的进化和结构。其中， HvTRE1-5在H. vigintioctopunctata四龄后的相对表达水平存在显着差异，并受到不同植物饮食的影响。与天然寄主黑龙江省相比，取食佛罗里达紫罗兰的幼虫体内海藻糖、葡萄糖和糖原的含量显着降低，海藻糖酶活性也受到显着影响。 RNAi 敲低HvTRE1-5会增加H. vigintioctopunctata化蛹阶段的死亡率，表明HvTRE1-5对于H. vigintioctopunctata化蛹很重要。这项研究为开发幽门螺杆菌绿色控制方法提供了新的见解。 vigintioctopunctata 并为理解宿主植物和草食性昆虫之间的相互作用提供了一个有价值的例子。