The present research aimed develop an innovative and efficient method for fabricating silver and palladium nanoparticles (NPs)by using exopolysaccharide (EPS) from the seaweed-associated -symbiotic bacteria (OP919469). Through, the application is a combined precipitation approach, EPS acts as a reducing and stabilising agent for the production of NPs. The major functional groups of NPs such as C–H, N–H, C–C, and C–O stretching’s were detected. The structural morphology of NPs showed a mixture of needle, spherical, and triangle-shaped with size between 10 and 50 nm. The purity of EPS was confirmed by high-performance liquid chromatography which revealed 99.81 % purity. The NPs revealed strong anti-cancer and antioxidant properties. In addition, the NPs have been shown to dose-dependent toxicity with reduced hatching, survival rate, and abnormalities in zebrafish. The biochemical assay revealed that the EPS loaded NPsdid not induce oxidative stress up to 120 hpf in contrast to hydrogen peroxide which had a significant effect () on glutathione-S-transferase, catalase ), and superoxide dismutase (). Overall, our finding indicates that the molecular and functional characteristics of EPS and EPS loaded NPs represent future potential applications in the drug delivery and healthcare sectors.

中文翻译：

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海洋微生物胞外多糖途径合成银和钯金属纳米粒子：可能的抗癌和抗氧化应用


本研究旨在开发一种创新且有效的方法，利用来自海藻相关共生细菌 (OP919469) 的胞外多糖 (EPS) 来制造银和钯纳米颗粒 (NP)。通过采用组合沉淀方法，EPS 充当 NP 生产的还原剂和稳定剂。检测到 NP 的主要官能团，例如 C-H、N-H、C-C 和 C-O 拉伸。纳米颗粒的结构形态呈现针状、球形和三角形的混合形状，尺寸在10至50 nm之间。 EPS的纯度经高效液相色谱法证实，纯度为99.81%。纳米粒子显示出强大的抗癌和抗氧化特性。此外，NPs 已被证明具有剂量依赖性毒性，会降低斑马鱼的孵化率、存活率和异常。生化测定显示，与过氧化氢相比，负载 EPS 的 NP 在 120 hpf 内不会诱导氧化应激，过氧化氢对谷胱甘肽-S-转移酶、过氧化氢酶和超氧化物歧化酶具有显着影响 ()。总体而言，我们的发现表明 EPS 和 EPS 负载的纳米粒子的分子和功能特征代表了药物输送和医疗保健领域的未来潜在应用。