The investigation within the green biorefinery focused on value-measuring properties of by-products derived from leaf protein concentrate production, particularly from Jerusalem artichokes. Results showed fiber fractions ranged from 17 to 28 % per 1 kg of fresh biomass, while brown juice fractions ranged from 57 to 68 %. Protein content varied from 7 to 14 m/m% for fiber and 0.3–1.4 m/m% for brown juice, with Rubik and Piri varieties exhibiting minimal differences in crude protein content between harvests. Amino acid analysis revealed significant amounts of leucine and lysine in both fractions. Phenolic compounds, notably hydroxy-methoxy flavones, were abundant in brown juice, with Rubik variety showing promising total phenolic content (TPC) and total flavonoid content (TFC). Qualitative determination identified chlorogenic acid and two isomers among cinnamic acid derivatives, suggesting potential medicine candidates. Brown juice fractions, especially from Rubik variety, hold promise for applications such as biostimulants. Further exploration of phenolic components in brown juice could yield valuable insights.

中文翻译：

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菊芋（Helianthus tuberosus L.）绿色生物质纤维和棕汁副产物的关键参数和植物化学成分的鉴定


绿色生物精炼厂的调查重点是叶浓缩蛋白生产副产品（尤其是菊芋）的价值测量特性。结果显示，每 1 公斤新鲜生物质的纤维含量为 17% 至 28%，而棕色果汁含量为 57% 至 68%。纤维的蛋白质含量为 7 至 14 m/m%，棕色果汁的蛋白质含量为 0.3–1.4 m/m%，Rubik 和 Piri 品种在收获之间的粗蛋白含量差异很小。氨基酸分析显示两个级分中均含有大量亮氨酸和赖氨酸。棕色果汁中含有丰富的酚类化合物，特别是羟基甲氧基黄酮，其中 Rubik 品种的总酚含量 (TPC) 和总黄酮含量 (TFC) 表现出良好的前景。定性测定鉴定出了绿原酸和肉桂酸衍生物中的两种异构体，表明了潜在的候选药物。棕色果汁馏分，尤其是来自 Rubik 品种的棕色果汁馏分，有望用于生物刺激剂等应用。对棕汁中酚类成分的进一步探索可能会产生有价值的见解。