Background and aims

Cereal-legume intercropping (IC) is proposed to address the challenges of increasing yields and improving crop nutrient quality, crucial for food security and human health. This study aimed to characterize the impact of pea-wheat IC on grain ionome composition, and asses its potential relation with the abundance of Pseudomonas spp. and Enterobacterales in plant roots.

Methods

In a field experiment, four pea varieties were cultivated in sole- or intercropping with wheat in two different soil types. Grain ionome was analysed by mass spectrometry, while Pseudomonas spp. and Enterobacterales abundances were quantified by qPCR.

Results

Pea grains intercropped with wheat showed increased concentrations of Ca, Mg, and Mo in one soil type, and higher Mn and Ni concentrations and total grain content in another. Wheat grains intercropped with peas, exhibited increased Cu, Fe, Mn, N, S, and Zn concentrations and/or total grain content, only in one soil type. Pseudomonas spp. showed increased abundance in pea root tissues when intercropped with wheat, specifically in one soil type. Pseudomonas spp. appeared to affect K, Fe, and Zn concentrations or total content in pea grains, depending on the cropping system.

Conclusion

These findings suggest that IC can enhance specific element concentrations and/or total grain content in pea and wheat grains, upon soil type. Pseudomonas spp. may facilitate nutrient uptake and translocation to grains. Further research is needed to understand the mechanisms behind element accumulation in IC grains and to explore the potential benefits of IC for plant nutrition and growth.

中文翻译：

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豌豆-小麦间作对籽粒离子组的影响与假单胞菌属的变化有关。和肠杆菌丰度

 背景和目标

谷类-豆类间作（IC）旨在解决提高产量和改善作物营养质量的挑战，这对粮食安全和人类健康至关重要。本研究旨在表征豌豆-小麦 IC 对谷物离子组组成的影响，并评估其与假单胞菌属丰度的潜在关系。和植物根部的肠杆菌目。

 方法

在田间试验中，四种豌豆品种在两种不同的土壤类型中与小麦单作或间作种植。通过质谱分析谷物离子组，而假单胞菌属。和肠杆菌丰度通过 qPCR 进行定量。

 结果

与小麦间作的豌豆籽粒显示，一种土壤类型中的钙、镁和钼浓度增加，而另一种土壤类型中的锰、镍浓度和谷物总含量更高。小麦粒与豌豆间作仅在一种土壤类型中表现出铜、铁、锰、氮、硫和锌浓度和/或谷物总含量增加。假单胞菌属当与小麦间作时，特别是在一种土壤类型中，豌豆根组织的丰度增加。假单胞菌属似乎会影响豌豆籽粒中钾、铁和锌的浓度或总含量，具体取决于种植制度。

 结论

这些发现表明，IC 可以根据土壤类型提高豌豆和小麦籽粒中的特定元素浓度和/或总籽粒含量。假单胞菌属可以促进养分的吸收和转移到谷物中。需要进一步的研究来了解 IC 谷物中元素积累的机制，并探索 IC 对植物营养和生长的潜在益处。