The study aimed to investigate the effect of supplementing polyclonal antibodies (PAP) of avian origin against the ruminal methanogens Methanobrevibacter gottschalkii Ho (PAP-Ho) and M. ruminantium M1 (PAP-M1) on ruminal fermentation profile and methane production in Angus crossbred cattle (13 steers and 1 heifer). The experiment was conducted using a randomized block design with a 3 × 2 + 1 factorial arrangement, replicated in 3 periods. The factors included proportions of PAP against Ho and M1 in the mixture (100:0, 50:50, and 0:100 Ho:M1) and level of each mixture (3- or 6-mL per d). Cattle in control treatment did not receive PAP supplementation. Ruminal fluid was collected from the animals on days 0, 14, and 21 of treatment to determine of ruminal fermentation profile and ex vivo methane production. There was no effect of level of inclusion on ex vivo methane production. Supplementation with PAP-M1, either alone or in combination with PAP-Ho, decreased ex vivo methane output compared to the control group. Furthermore, in vivo molar proportion of propionate tended to be greater with PAP-M1, alone or combined with PAP-Ho, when compared with the control group. The study concluded that polyclonal antibodies against ruminal methanogens have the potential to decrease enteric methane emissions in cattle. The research provided important insights into the potential use of PAP as a strategy for reducing greenhouse gas emissions from cattle. Further research is needed to confirm these findings and to determine the practicality and feasibility of using PAP.

中文翻译：

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补充针对 Methanobrevibacter gottschalkii 和 M. ruminantium 的禽源性多克隆抗体可减少安格斯杂交公牛离体甲烷的产生并改变瘤胃发酵


该研究旨在探讨补充针对瘤胃产甲烷菌 Methanobrevibacter gottschalkii Ho （PAP-Ho） 和 M. ruminantium M1 （PAP-M1） 的禽源性多克隆抗体 （PAP） 对安格斯杂交牛 （13 头公牛和 1 头小母牛） 瘤胃发酵曲线和甲烷产生的影响。该实验采用随机区组设计进行，采用 3 × 2 + 1 因子排列，分 3 个周期重复。这些因素包括混合物中 PAP 对 Ho 和 M1 的比例 （100：0、50：50 和 0：100 Ho：M1） 和每种混合物的水平 （3 mL 或 6 mL / d）。对照治疗中的牛未接受 PAP 补充剂。在治疗的第 0 天、第 14 天和第 21 天从动物身上收集瘤胃液，以确定瘤胃发酵概况和离体甲烷产生。包合水平对离体甲烷产生没有影响。与对照组相比，单独补充 PAP-M1 或与 PAP-Ho 联合补充可降低离体甲烷输出量。此外，与对照组相比，PAP-M1 单独使用或与 PAP-Ho 联合使用丙酸盐的体内摩尔比例往往更高。该研究得出结论，针对瘤胃产甲烷菌的多克隆抗体有可能减少牛的肠道甲烷排放。该研究为将 PAP 用作减少牛温室气体排放的策略提供了重要见解。需要进一步的研究来证实这些发现并确定使用 PAP 的实用性和可行性。