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A single-molecule fluorescent probe for visualizing viscosity and hypoxia in lysosomes and zebrafish embryos
Analyst ( IF 3.6 ) Pub Date : 2024-08-10 , DOI: 10.1039/d4an00906a Jingchao Wang 1 , Lina Zhou 1 , Zekun Jiang 1 , Haiyan Wu 2 , Xiuqi Kong 1
Analyst ( IF 3.6 ) Pub Date : 2024-08-10 , DOI: 10.1039/d4an00906a Jingchao Wang 1 , Lina Zhou 1 , Zekun Jiang 1 , Haiyan Wu 2 , Xiuqi Kong 1
Affiliation
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Viscosity and hypoxia, as microenvironment parameters, play important roles in maintaining normal biological processes and homeostasis. Therefore, simultaneous and sensitive detection of these elements with simple and effective methods could offer precise information in biology. Here, we report a two-site lysosome-targeting fluorescent probe, NVP, for monitoring viscosity and nitroreductase with dual emission channels (emission shift is 86 nm). The NVP probe has displayed highly sensitive and selective responses towards viscosity and nitroreductase, respectively. Significantly, the fluctuations of viscosity and NTR have been detected in vitro and in vivo. We expect that the dual-responsive fluorescent NVP probe will become a potential molecular tool for the exploration of deeper functions of viscosity and hypoxia.
中文翻译：
用于可视化溶酶体和斑马鱼胚胎中粘度和缺氧的单分子荧光探针
粘度和缺氧作为微环境参数，在维持正常的生物过程和体内平衡中发挥着重要作用。因此，用简单有效的方法同时灵敏地检测这些元素可以提供精确的生物学信息。在这里，我们报告了一种双位点溶酶体靶向荧光探针NVP ，用于监测粘度和具有双发射通道的硝基还原酶（发射位移为 86 nm）。 NVP探针分别对粘度和硝基还原酶表现出高度灵敏和选择性的响应。值得注意的是，在体外和体内都检测到了粘度和 NTR 的波动。我们期望双响应荧光NVP探针将成为探索粘性和缺氧更深层次功能的潜在分子工具。
更新日期：2024-08-10
中文翻译：
用于可视化溶酶体和斑马鱼胚胎中粘度和缺氧的单分子荧光探针
粘度和缺氧作为微环境参数，在维持正常的生物过程和体内平衡中发挥着重要作用。因此，用简单有效的方法同时灵敏地检测这些元素可以提供精确的生物学信息。在这里，我们报告了一种双位点溶酶体靶向荧光探针NVP ，用于监测粘度和具有双发射通道的硝基还原酶（发射位移为 86 nm）。 NVP探针分别对粘度和硝基还原酶表现出高度灵敏和选择性的响应。值得注意的是，在体外和体内都检测到了粘度和 NTR 的波动。我们期望双响应荧光NVP探针将成为探索粘性和缺氧更深层次功能的潜在分子工具。
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