Ultra-high static magnetic fields altered the embryonic division and development in Caenorhabditis elegans via multipolar spindles,Journal of Advanced Research

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Ultra-high static magnetic fields altered the embryonic division and development in Caenorhabditis elegans via multipolar spindles
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2024-07-31 , DOI: 10.1016/j.jare.2024.07.032
Lei Cheng ₁ , Mudi Wang ₂ , Baolin Yang ₁ , Yang Li ₁ , Tong Wang ₃ , Chuanying Xi ₄ , Yuyan Han ₄ , Ze Wang ₄ , Yanwen Fang ₅ , Min Wei ₅ , Hua Du ₃ , An Xu ₁

Affiliation

Introduction

Ultra-high static magnetic fields (SMFs) have unique advantages in improving medical and academic research. However, the research on the early embryo exposure of ultra-high SMFs is minimal, extensive exploration is indispensable in living organisms.

Objectives

The present study was aimed to study the effects of ultra-high SMFs on the early embryonic division and development of Caenorhabditis elegans (C. elegans).

Methods

Early adult parents containing fertilized eggs in vivo were exposed to SMFs at intensities ranging from 4 T to 27 T. The number of mitotic cells in the reproductive glands of the P0 worms, early embryonic cell spindle localization, embryo hatching and the reproductive as well as developmental indicators of F1 and F2 nematodes were examined as endpoints.

Results

Our results indicated that ultra-high SMFs has no obvious effect on the germ cell cycle, while 14 T and 27 T SMFs significantly increased the proportion of multi-polar spindle formation in early embryonic cells, and reduced the developmental rate and lifespan of C. elegans exposed at the embryonic stage. Spindle abnormalities of early embryonic cells, as well as the down-regulation of genes related to asymmetric embryonic division and the abnormal expression of the non-muscle myosin NMY-2 in the division grooves played a critical role in the slowing down of embryonic development induced by ultra-high SMFs.

Conclusions

This study provided novel information and a new sight for evaluating the biosafety assessment by exposure to ultra-high SMFs at the early embryonic stage in vivo.

中文翻译：

超高静电场通过多极纺锤体改变了秀丽隐杆线虫的胚胎分裂和发育

介绍

超高静电场（SMF）在改善医学和学术研究方面具有独特的优势。然而，对超高 SMFs 的早期胚胎暴露的研究很少，在生物体中广泛探索是必不可少的。

目标

本研究旨在研究超高 SMFs 对秀丽隐杆线虫（C. elegans）早期胚胎分裂和发育的影响。

方法

体内含有受精卵的早期成年父母暴露于强度为 4 T 至 27 T 的 SMF。以 P0 蠕虫生殖腺中有丝分裂细胞的数量、早期胚胎细胞梭体定位、胚胎孵化以及 F1 和 F2 线虫的生殖和发育指标为终点。

结果

我们的结果表明，超高 SMFs 对生殖细胞周期没有明显影响，而 14 T 和 27 T SMFs 显著增加了早期胚胎细胞中多极纺锤体形成的比例，降低了胚胎期暴露的秀丽隐杆线虫的发育速度和寿命。早期胚胎细胞的纺锤体异常，以及与不对称胚胎分裂相关的基因的下调和分裂沟中非肌肉肌球蛋白 NMY-2 的异常表达在超高 SMFs 诱导的胚胎发育减慢中起关键作用。