The current study aimed to explore the genotoxic impacts of the insecticide acetamiprid (ACP) on the myocardium and assess the ameliorative role of resveratrol (RSV). Male rats (10/group) were treated via oral route for 90 days: control; ACP (25 mg/kg); RSV (20 mg/kg); ACP+RSV. Peripheral blood micronucleus test, oxidative stress analysis, comet assay, 8-hydroxydeoxyguanosine and gene expression assessment were performed. The findings revealed that ACP has myocardial genotoxic effects, as demonstrated by increased micronucleus and 8-hydroxydeoxyguanosine formation and increased all comet parameters. Oxidative stress analysis demonstrated that ACP elevated HO and NO levels while decreasing catalase and GST activities. Acetamiprid dysregulated the expression of genes related to oxidative stress and DNA damage response. However, RSV co-treatment resulted in significant protection against these genotoxic impacts. Resveratrol reduced DNA damage and restored the oxidative balance in the myocardium. Moreover, RSV modulated the Nrf2/HO-1 and Atm/P53 pathways, potentiating antioxidant defense and DNA repair.

中文翻译：

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啶虫脒引起的心肌遗传毒性的机制和白藜芦醇的潜在改善作用


本研究旨在探讨杀虫剂啶虫脒 (ACP) 对心肌的基因毒性影响，并评估白藜芦醇 (RSV) 的改善作用。雄性大鼠（10只/组）经口给药90天：对照组； ACP（25 毫克/千克）； RSV（20毫克/千克）； ACP+RSV。进行外周血微核测试、氧化应激分析、彗星试验、8-羟基脱氧鸟苷和基因表达评估。研究结果表明，ACP 具有心肌遗传毒性作用，这通过增加微核和 8-羟基脱氧鸟苷形成以及增加所有彗星参数来证明。氧化应激分析表明，ACP 升高 H2O 和 NO 水平，同时降低过氧化氢酶和 GST 活性。啶虫脒可调节与氧化应激和 DNA 损伤反应相关的基因表达。然而，RSV 共同治疗可显着防止这些遗传毒性影响。白藜芦醇减少 DNA 损伤并恢复心肌的氧化平衡。此外，RSV 还调节 Nrf2/HO-1 和 Atm/P53 通路，增强抗氧化防御和 DNA 修复。