A DNA origami nanocompartment is designed to trap an unfoldase machine in a unidirectional orientation. This trapping provides a gateway mechanism for substrate recruitment and translocation to a downstream compartment that hosts a protease. Kinetics and proteomics data demonstrate that the physical connection of the DNA-based modules improves the global performance of the chimera and reduces off-target reactions.

中文翻译：

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使用 DNA 折纸设计模块化酶


DNA 折纸纳米隔室旨在将 unfoldase 机器捕获在单向方向。这种捕获为底物募集和易位到承载蛋白酶的下游隔室提供了一种网关机制。动力学和蛋白质组学数据表明，基于 DNA 的模块的物理连接提高了嵌合体的整体性能并减少了脱靶反应。