A condensates-to-VPS41-associated phagic vacuoles conversion pathway controls autophagy degradation in plants,Developmental Cell

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A condensates-to-VPS41-associated phagic vacuoles conversion pathway controls autophagy degradation in plants
Developmental Cell ( IF 10.7 ) Pub Date : 2024-08-06 , DOI: 10.1016/j.devcel.2024.07.010
Dong Jiang ₁ , Yilin He ₁ , Hailin Li ₁ , Liufeng Dai ₂ , Bingyan Sun ₁ , Lianming Yang ₁ , Lei Pang ₁ , Zhiran Cao ₁ , Yu Liu ₁ , Jiayang Gao ₃ , Yi Zhang ₂ , Liwen Jiang ₄ , Ruixi Li ₁

Affiliation

Autophagy is a universal degradation system in eukaryotic cells. In plants, although autophagosome biogenesis has been extensively studied, the mechanism of how autophagosomes are transported to the vacuole for degradation remains largely unexplored. In this study, we demonstrated that upon autophagy induction, homotypic fusion and protein sorting (HOPS) subunit VPS41 converts first from condensates to puncta, then to ring-like structures, termed VPS41-associated phagic vacuoles (VAPVs), which enclose autophagy-related gene (ATG)8s for vacuolar degradation. This process is initiated by ADP ribosylation factor (ARF)-like GTPases ARLA1s and occurs concurrently with autophagy progression through coupling with the synaptic-soluble N-ethylmaleimide-sensitive factor attachment protein rmleceptor (SNARE) proteins. Unlike in other eukaryotes, autophagy degradation in is largely independent of the RAB7 pathway. By contrast, dysfunction in the condensates-to-VAPVs conversion process impairs autophagosome structure and disrupts their vacuolar transport, leading to a significant reduction in autophagic flux and plant survival rate. Our findings suggest that the conversion pathway might be an integral part of the autophagy program unique to plants.

中文翻译：

冷凝物到 VPS41 相关的吞噬泡转化途径控制植物中的自噬降解

自噬是真核细胞中普遍存在的降解系统。在植物中，尽管自噬体的生物发生已被广泛研究，但自噬体如何转运至液泡进行降解的机制在很大程度上仍未被探索。在这项研究中，我们证明，在自噬诱导后，同型融合和蛋白质分选（HOPS）亚基VPS41首先从凝结物转化为点状结构，然后转化为环状结构，称为VPS41相关吞噬泡（VAPV），其包围自噬相关的液泡降解基因 (ATG)8s。该过程由 ADP 核糖基化因子 (ARF) 样 GTPases ARLA1 启动，并通过与突触可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体 (SNARE) 蛋白偶联而与自噬进程同时发生。与其他真核生物不同，自噬降解在很大程度上独立于 RAB7 途径。相比之下，凝结物向 VAPV 转化过程中的功能障碍会损害自噬体结构并扰乱其液泡运输，导致自噬通量和植物存活率显着降低。我们的研究结果表明，转化途径可能是植物特有的自噬程序的一个组成部分。

更新日期：2024-08-06

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