<br>基于UHPLC-Q-Orbitrap HRMS的文冠果种子不同部位化学成分比较,China Journal of Chinese Materia Medica

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基于UHPLC-Q-Orbitrap HRMS的文冠果种子不同部位化学成分比较
China Journal of Chinese Materia Medica Pub Date : 2023-12-01 , DOI: 10.19540/j.cnki.cjcmm.20230904.101
Jun Yuan ₁ , Hong-Wei Zhang ₁ , Zhen-Ling Zhang ₂ , Ya-Ning Wu ₁ , Ya-Jing Li ₁

Affiliation

文冠果种子在食品和制药行业有着广泛的应用。为比较分析文冠果种子不同部位的化学成分，探讨文冠果非药用部位的潜在价值和研究前景，本研究采用超高效液相色谱四极杆 Orbitrap 高分辨率质谱仪（UHPLC-Q） -Orbitrap HRMS）检测种子各部分的化学成分。文冠果种子中初步鉴定出82种成分，其中氨基酸5种、多酚4种、苯丙酸3种、有机酸7种、黄酮类15种、苷类6种、皂苷23种。对种子不同部位的结果进行质谱分子网络（MN）分析，发现种子仁、种皮和种壳的成分存在显着差异。种仁中的皂苷和黄酮类化合物的种类和含量均优于种皮和壳中的皂苷和黄酮类化合物。根据多批次样品不同部位的色谱峰，进行多元统计分析。采用高效液相色谱法测定了4种差异成分，其在种仁、种皮、种壳中的平均含量分别为：白蜡花素0.183 6、0.887 4、1.440 1 mg·g~(-1)；儿茶素为0.035 8、0.124 1、0.044 5 mg·g~(-1)；弗拉西丁为0.032 9、0.072 0、0.221 5 mg·g~(-1)；表儿茶素为0.435 9、2.114 7、0.259 7 mg·g~(-1)。结果表明，儿茶素和白蜡黄素在各部位含量较低，白蜡黄素在种皮和种壳中含量较高，表儿茶素在种仁和种皮中含量较高。因此，种皮和种壳具有一定的开发价值。本研究对文冠果种子不同部位的化学成分进行了快速分析和比较，为文冠果种子药用物质的研究和临床应用提供了实验基础。

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[Comparison of chemical compositions of different parts of Xanthoceras sorbifolium seeds based on UHPLC-Q-Orbitrap HRMS].

Xanthoceras sorbifolium seeds have a wide range of applications in the food and pharmaceutical industries. To compare and analyze the chemical compositions of different parts of X. sorbifolium seeds and explore the potential value and research prospects of non-medicinal parts, this study used ultra-high-performance liquid chromatography quadrupole Orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS) to detect the chemical composition of various parts of the seeds. A total of 82 components were preliminary identified from X. sorbifolium seeds, including 5 amino acids, 4 polyphenols, 3 phenylpropionic acids, 7 organic acids, 15 flavonoids, 6 glycosides, and 23 saponins. Mass spectrometry molecular networking(MN) analysis was conducted on the results from different parts of the seeds, revealing significant differences in the components of the seed kernel, seed coat, and seed shell. The saponins and flavonoids in the seed kernel were superior in terms of variety and content to those in the seed coat and shell. Based on the chromatographic peaks of different parts from multiple batches of samples, multivariate statistical analysis was carried out. Four differential components were determined using HPLC, and the average content of these components in the seed kernel, seed coat, and seed shell were as follows: 0.183 6, 0.887 4, and 1.440 1 mg·g~(-1) for fraxin; 0.035 8, 0.124 1, and 0.044 5 mg·g~(-1) for catechin; 0.032 9, 0.072 0, and 0.221 5 mg·g~(-1) for fraxetin; 0.435 9, 2.114 7, and 0.259 7 mg·g~(-1) for epicatechin. The results showed that catechin and fraxetin had relatively low content in all parts, while fraxin had higher content in the seed coat and seed shell, and epicatechin had higher content in the seed kernel and seed coat. Therefore, the seed coat and seed shell possess certain development value. This study provides rapid analysis and comparison of the chemical compositions of different parts of X. sorbifolium seeds, which offers an experimental basis for the research and clinical application of medicinal substances in X. sorbifolium seeds.

更新日期：2023-12-01

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