Brewer’s dried yeast has a high nutritional value and has long been utilized by the animal feed industry as a source of protein, B-complex vitamins, and minerals. Brewer’s dried yeast is also rich in bioactive compounds and may thereby be used as a functional ingredient, providing benefits beyond that of its nutrient content. Canola meal is a high-fiber ingredient that also has unique properties, especially when it is wetted and dried using a proprietary drying system that creates a “functionalized” canola meal. The objective of this experiment was to evaluate the effects of a yeast-enriched functionalized canola meal (FCM) on apparent total tract digestibility (ATTD) and the fecal quality, metabolite concentrations, and microbiota populations, and immune function of healthy adult dogs. Twelve adult female beagles [body weight (BW) = 7.6 ± 0.7 kg; age = 5.8 ± 1.3] were used in a replicated 4×4 Latin square design with 28-d periods. Each experimental period consisted of a 22-d adaptation phase, 5 days of total and fresh fecal collection, and blood collection on the last day. To start, all dogs were fed a basal diet to maintain BW for 14 d. Following fecal and blood collections at baseline (-1 d) to confirm health status, experimental periods began testing the following dietary treatments using a Latin square design experiment: 1) FCM only (no yeast inclusion), 2) FCM + low yeast dose, 3) FCM + medium yeast dose, and 4) FCM + high yeast dose. All treatments were top-dressed onto the basal diet at a rate estimated to be 1% of daily intake (as-is basis). Statistical analysis was performed using the PROC MIXED procedure of SAS with the main effect of treatment and the random effect of dog. Significance was declared at P ≤ 0.05, and trends reported if 0.05 < P ≤ 0.10. Supplementation with yeast-enriched FCM had no significant effect on the ATTD of macronutrients or energy or the fecal characteristics, metabolite concentrations, and microbiota populations of dogs. Additionally, no significant differences were observed in circulating immune cell counts or response to Toll-like receptor agonists among treatments. Our results suggest that the yeast-enriched FCM could be included in canine diets without negatively affecting stool quality, fecal metabolite concentrations, or ATTD. Further research is necessary to determine the effective dose of yeast-enriched FCM, potential mechanisms of action, and other potential implications it has on canine health.

中文翻译：

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富含酵母的功能化菜籽油粉补充剂对健康成年犬表观总道常量营养素消化率和粪便特性、粪便微生物群和免疫功能的影响


Brewer 的干酵母具有很高的营养价值，长期以来一直被动物饲料行业用作蛋白质、B 族复合维生素和矿物质的来源。啤酒干酵母还富含生物活性化合物，因此可用作功能性成分，提供超出其营养成分的好处。菜籽粕是一种高纤维成分，也具有独特的特性，尤其是当它使用专有的干燥系统进行润湿和干燥时，会产生“功能化”的菜籽粕。本实验的目的是评估富含酵母的功能化菜籽油粉 （FCM） 对健康成年犬表观总消化率 （ATTD） 和粪便质量、代谢物浓度和微生物群群以及免疫功能的影响。12 只成年雌性比格犬 [体重 （BW） = 7.6 ± 0.7 kg;年龄 = 5.8 ± 1.3] 用于复制的 4×4 拉丁方设计，周期为 28-d。每个实验期包括 22 天适应阶段、5 天的总粪便和新鲜粪便收集以及最后一天的血液采集。首先，所有狗都喂食基础饮食以维持 BW 14 天。在基线 （-1 d） 采集粪便和血液以确认健康状况后，实验期开始使用拉丁方设计实验测试以下饮食处理：1） 仅 FCM（无酵母包涵），2） FCM + 低酵母剂量，3） FCM + 中等酵母剂量，以及 4） FCM + 高酵母剂量。所有治疗均以估计为每日摄入量的 1% 的速率（按原样）在基础饮食中施肥。使用 SAS 的 PROC MIXED 程序进行统计分析，治疗的主要效果和狗的随机效果。在 P ≤ 0.05 时宣布显著性，如果 0.05 < P ≤ 0.10，则报告趋势。 补充富含酵母的 FCM 对狗的宏量营养素或能量的 ATTD 或粪便特性、代谢物浓度和微生物群群没有显着影响。此外，在处理之间未观察到循环免疫细胞计数或对 Toll 样受体激动剂的反应存在显著差异。我们的结果表明，富含酵母的 FCM 可以包含在犬饮食中，而不会对粪便质量、粪便代谢物浓度或 ATTD 产生负面影响。需要进一步的研究来确定富含酵母的 FCM 的有效剂量、潜在的作用机制以及它对犬类健康的其他潜在影响。