The sequalae of periodontitis include irreversible degradation of tooth-supporting structures and circulatory spread of inflammatory mediators. However, the serum protein profile in periodontitis is not well described, which is partly attributable to the limited number of studies based on large and well-characterized periodontitis cohorts. This study aims to identify novel, circulating inflammation-related proteins associated with periodontitis within the PerioGene North case-control study, which includes 478 cases with severe periodontitis and 509 periodontally healthy controls. The serum concentrations of high-sensitivity C-reactive protein (hs-CRP) and a panel of 45 inflammation-related proteins were analyzed using targeted proteomics. A distinguishable serum protein profile was evident in periodontitis cases. The protein pattern could separate cases from controls with a sensitivity of 0.81 and specificity of 0.81 (area under the curve = 0.87). Adjusted levels for hs-CRP and 24 of the 45 proteins were different between cases and controls. High levels of hs-CRP and matrix metalloproteinase–12, and low levels of epidermal growth factor (EGF) and oxidized low-density lipoprotein receptor 1 (OLR-1) were detected among the cases. Furthermore, the levels of C-C motif chemokine–19, granulocyte colony-stimulating factor–3 (CSF-3), interleukin-7 (IL-7), and hs-CRP were significantly higher in cases with a high degree of gingival inflammation. The levels of CSF-3 and tumor necrosis factor ligand superfamily member–10 TNFSF-10 were higher in cases with many deep periodontal pockets. The PerioGene North study includes detailed clinical periodontal data and uncovers a distinct serum protein profile in periodontitis. The findings of lower EGF and OLR-1 among the cases are highlighted, as this has not been presented before. The role of EGF and OLR-1 in periodontitis pathogenesis and as possible future biomarkers should be further explored.

中文翻译：

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PerioGene North 研究发现了与牙周炎相关的血清蛋白


牙周炎的后遗症包括牙齿支持结构的不可逆退化和炎症介质的循环扩散。然而，牙周炎的血清蛋白谱尚未得到很好的描述，部分原因是基于大型且特征明确的牙周炎队列的研究数量有限。本研究旨在在 PerioGene North 病例对照研究中鉴定与牙周炎相关的新型循环炎症相关蛋白，该研究包括 478 例严重牙周炎病例和 509 例牙周健康对照病例。使用靶向蛋白质组学分析了高敏 C 反应蛋白 (hs-CRP) 和一组 45 种炎症相关蛋白的血清浓度。牙周炎病例中明显存在明显的血清蛋白谱。蛋白质模式可以以 0.81 的敏感性和 0.81 的特异性（曲线下面积 = 0.87）区分病例和对照。 hs-CRP 和 45 种蛋白质中的 24 种的调整后水平在病例和对照之间存在差异。病例中检测到高水平的hs-CRP和基质金属蛋白酶-12，以及低水平的表皮生长因子（EGF）和氧化低密度脂蛋白受体1（OLR-1）。此外，在牙龈炎症程度较高的病例中，CC 基序趋化因子-19、粒细胞集落刺激因子-3 (CSF-3)、白细胞介素-7 (IL-7) 和 hs-CRP 的水平显着升高。在牙周袋较多的病例中，CSF-3 和肿瘤坏死因子配体超家族成员 10 TNFSF-10 的水平较高。 PerioGene North 研究包括详细的临床牙周数据，并揭示了牙周炎中独特的血清蛋白谱。 强调了病例中 EGF 和 OLR-1 较低的发现，因为这是以前从未提出过的。 EGF 和 OLR-1 在牙周炎发病机制中的作用以及未来可能的生物标志物应进一步探讨。