This study was investigated to optimize growth conditions of native microalgae (Coelastrella sp. CORE-3) from wastewater, evaluate the removal of Cu2+, Zn2+, Cd2+ and Ni2+ and compare both isolated microalgae and Ankistrodesmus bibraianus, and describe their sorption mechanisms on the cell surface. The effects of the pH, light: dark cycle and temperature on the growth of the isolated Coelastrella sp. CORE-3 was optimized. The optimal L:D cycle, temperature, and pH conditions were 14:10 hr, 25℃, pH 7. The growth of isolated microalgae, Coelastrella sp. CORE-3. was compared with A. bibraianus. The removal efficiency of heavy metals by Coelastrella sp. CORE-3 and A. bibraianus were found as follows, respectively: (live cell) Zn2+ > Cd2+ > Cu2+ > Ni2+, (dead cell) Cu2+ > Cd2+ > Zn2+ > Ni2+. SEM analysis indicated that more cell surface deformation occurred in A. bibraianus compared to Coelastrella sp. after exposure to heavy metals. This result suggests that Coelastrella sp. CORE-3 has more resistant to heavy metal toxicity. In addition, based on the FT-IR analysis, the live cells of Coelastrella sp. CORE-3 and A. bibraianus showed the interactions of heavy metals with the functional groups: alcohol, alkane, amide, amine on their cell surfaces.

中文翻译：

---

Coelastrella sp.的最佳生长条件和重金属去除。从废水中分离出 CORE-3


本研究旨在优化废水中原生微藻（Coelastrella sp. CORE-3）的生长条件，评估 Cu2+、Zn2+、Cd2+ 和 Ni2+ 的去除效果，比较分离的微藻和 Ankistrodesmus bibraianus，并描述它们在细胞上的吸附机制表面。 pH、光：暗循环和温度对分离的 Coelastrella sp 生长的影响。 CORE-3 进行了优化。最佳L:D循环、温度和pH条件为14:10小时、25℃、pH 7。分离微藻Coelastrella sp的生长。核心-3。与 A. bibraianus 进行了比较。 Coelastrella sp.对重金属的去除效率。 CORE-3和A. bibraianus的发现分别如下：（活细胞）Zn2+ > Cd2+ > Cu2+ > Ni2+，（死细胞）Cu2+ > Cd2+ > Zn2+ > Ni2+。 SEM 分析表明，与 Coelastrella sp 相比，A. bibraianus 发生了更多的细胞表面变形。接触重金属后。该结果表明Coelastrella sp。 CORE-3对重金属毒性具有更强的抵抗力。此外，根据 FT-IR 分析，Coelastrella sp. 的活细胞。 CORE-3 和 A. bibraianus 显示了重金属与其细胞表面的醇、烷、酰胺、胺等官能团的相互作用。